Objective: To evaluate the frequency of autoantibodies (Ab) against 21 hydroxylase (210H), side-chain cleavage (SCC) and 17α-hydroxylase (170H), in Addison's disease (AD) and autoimmune polyendocrine syndrome type III (APSIII). Design and Methods: We used radiobinding assays and in vitro translated recombinant human 35S-210H, 35S-SCC or 35S-170H and studied serum samples from 29 AD (18 idiopathic, 11 granulomatous) and 18 APSIII (autoimmune thyroid disease plus type 1 diabetes mellitus, without AD) patients. Results were compared with those of adrenocortical autoantibodies obtained with indirect immunofluorescence (ACA-IIF). Results: ACA-IIF were detected in 15/18 (83%) idiopathic and in 1/11 (9%) granulomatous AD subjects. 210HAb were found in 14/18 (78%) idiopathic and in the same (9%) granulomatous AD subject. A significant positive correlation was shown between ACA-IIF and 210HAb levels (r2 = 0.56, P < 0.02). The concordance rate between the two assays was 83% (24/29) in AD patients. SCCAb were found in 5/18 (28%) idiopathic (4 of whom were also positive for 210HAb) and in the same (9%) granulomatous AD subject. 170HAb were found in only 2/18 (11%) idiopathic and none of the granulomatous AD patients. Two APSIII patients were positive for ACA-IIF, but only one was positive for 210HAb and SCCAb. 170HAb were found in another two APSIII patients. Conclusions: Measurement of 210HAb should be the first step in immune assessment of patients with AD and individuals at risk for adrenal autoimmunity, in addition to ACA-IIF. Due to their low prevalence in AD, measurement of SCCAb and 170HAb should be indicated only for 210HAb negative patients and/or for those with premature ovarian failure, regardless of ACA-IIF results.

Autoantibodies against recombinant human steroidogenic enzymes 21-hydroxylase, side-chain cleavage and 17alpha-hydroxylase in Addison’s disease and autoimmune polyendocrine syndrome type III

FALORNI, Alberto
2000

Abstract

Objective: To evaluate the frequency of autoantibodies (Ab) against 21 hydroxylase (210H), side-chain cleavage (SCC) and 17α-hydroxylase (170H), in Addison's disease (AD) and autoimmune polyendocrine syndrome type III (APSIII). Design and Methods: We used radiobinding assays and in vitro translated recombinant human 35S-210H, 35S-SCC or 35S-170H and studied serum samples from 29 AD (18 idiopathic, 11 granulomatous) and 18 APSIII (autoimmune thyroid disease plus type 1 diabetes mellitus, without AD) patients. Results were compared with those of adrenocortical autoantibodies obtained with indirect immunofluorescence (ACA-IIF). Results: ACA-IIF were detected in 15/18 (83%) idiopathic and in 1/11 (9%) granulomatous AD subjects. 210HAb were found in 14/18 (78%) idiopathic and in the same (9%) granulomatous AD subject. A significant positive correlation was shown between ACA-IIF and 210HAb levels (r2 = 0.56, P < 0.02). The concordance rate between the two assays was 83% (24/29) in AD patients. SCCAb were found in 5/18 (28%) idiopathic (4 of whom were also positive for 210HAb) and in the same (9%) granulomatous AD subject. 170HAb were found in only 2/18 (11%) idiopathic and none of the granulomatous AD patients. Two APSIII patients were positive for ACA-IIF, but only one was positive for 210HAb and SCCAb. 170HAb were found in another two APSIII patients. Conclusions: Measurement of 210HAb should be the first step in immune assessment of patients with AD and individuals at risk for adrenal autoimmunity, in addition to ACA-IIF. Due to their low prevalence in AD, measurement of SCCAb and 170HAb should be indicated only for 210HAb negative patients and/or for those with premature ovarian failure, regardless of ACA-IIF results.
2000
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/10941
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