Oxytetracycline hydrochloride (OTC) belongs to the Tetracyclines class and it is one of the most widely used drugs for the treatment of bacterial infections in fish. OTC is poorly absorbed when administered with food, because it forms complexes with metal ions present in feed. However when the administered dose is too high, it is possible to find residues in food, which can cause immunosuppressive effects and bacterial resistance. The aim of this work is to simulate the pharmacological treatment with OTC, contaminating the feed from therapeutic levels, suitable for the species Cyprinus carpio to higher doses, to obtain data relating to carry-over in muscle and liver. 160 carps has been divided into 8 pools with a continuous flow of lake water and acclimated for 5 days. The fish were fed for 10 days with medicated feed, the antibiotic was administered to 1.5% bw day at doses of 75 (recommended dose), 150 and 300 mg/kg of body weight. Before administration 8 specimens were randomly sampled, other 6 fish per tank were sampled at the end of the 10 days of dosing and 5 and 10 days after the end of treatment. The muscle was collected for the antibiotic determination using an high performance liquid chromatography with diode array detector (HPLC-DAD) method .
Farmaci nei mangimi impiegati in acquacoltura: valutazione della sicurezza nell’uso di ossitetraciclina
ELIA, Antonia Concetta;DOERR, Ambrosius J. Martin;
2012
Abstract
Oxytetracycline hydrochloride (OTC) belongs to the Tetracyclines class and it is one of the most widely used drugs for the treatment of bacterial infections in fish. OTC is poorly absorbed when administered with food, because it forms complexes with metal ions present in feed. However when the administered dose is too high, it is possible to find residues in food, which can cause immunosuppressive effects and bacterial resistance. The aim of this work is to simulate the pharmacological treatment with OTC, contaminating the feed from therapeutic levels, suitable for the species Cyprinus carpio to higher doses, to obtain data relating to carry-over in muscle and liver. 160 carps has been divided into 8 pools with a continuous flow of lake water and acclimated for 5 days. The fish were fed for 10 days with medicated feed, the antibiotic was administered to 1.5% bw day at doses of 75 (recommended dose), 150 and 300 mg/kg of body weight. Before administration 8 specimens were randomly sampled, other 6 fish per tank were sampled at the end of the 10 days of dosing and 5 and 10 days after the end of treatment. The muscle was collected for the antibiotic determination using an high performance liquid chromatography with diode array detector (HPLC-DAD) method .I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.