STRs are today one of the most effective tools for individual and populational genetic characterization. This paper presents the results of a population study of 15 STR loci included in AmpFlSTR Identifiler kit (Applied Biosystems) that has the aim of creating a local database. Blood or oral swab samples were obtained from 100 unrelated individuals, who were born in Terni (in the center of Italy) and lived there for at least two generations. DNA was extracted from blood, using QIAamp, miniKit of Qiagen and from oral swab, using Chelex method. For each locus allele frequency was calculated and Hardy-Weinberg equilibrium was evaluated. The Expected Heterozygosity (He), the Observed Heterozygosity (Ho), the Polymorphic Information Content (PIC), the Power of Discrimination (PD) and the Power of exclusion (PE) were also calculated.
Allele frequencies of 15 STR loci in an Italian population
LANCIA, MASSIMO;BACCI, Mauro
2006
Abstract
STRs are today one of the most effective tools for individual and populational genetic characterization. This paper presents the results of a population study of 15 STR loci included in AmpFlSTR Identifiler kit (Applied Biosystems) that has the aim of creating a local database. Blood or oral swab samples were obtained from 100 unrelated individuals, who were born in Terni (in the center of Italy) and lived there for at least two generations. DNA was extracted from blood, using QIAamp, miniKit of Qiagen and from oral swab, using Chelex method. For each locus allele frequency was calculated and Hardy-Weinberg equilibrium was evaluated. The Expected Heterozygosity (He), the Observed Heterozygosity (Ho), the Polymorphic Information Content (PIC), the Power of Discrimination (PD) and the Power of exclusion (PE) were also calculated.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
