The present paper describes the most recent advances in the encapsulation of Sertoli's cells (SC) for in vivo transplantation. Recent reports have demonstrated that Sertoli's cells may improve both in vitro and in vivo functional competence of different cells; in particular, SC improve, in vitro, the functional competence of pancreatic islets (I) and, in vivo, prolong islet allographt survival in animal model of diabetes mellitus. We have developed a method for high yield separation of purified SC from rodents and porcine pre-pubertal testes and different methods for the encapsulation of SC in alginate microcapsules, namely, "Medium size" (MSM) and "Coherent" Microcapsules (CM).
Encapsulation of Sertoli's Cells For In Vivo Transplantation.
LUCA, Giovanni;CALAFIORE, Riccardo;RICCI, Maurizio;
2000
Abstract
The present paper describes the most recent advances in the encapsulation of Sertoli's cells (SC) for in vivo transplantation. Recent reports have demonstrated that Sertoli's cells may improve both in vitro and in vivo functional competence of different cells; in particular, SC improve, in vitro, the functional competence of pancreatic islets (I) and, in vivo, prolong islet allographt survival in animal model of diabetes mellitus. We have developed a method for high yield separation of purified SC from rodents and porcine pre-pubertal testes and different methods for the encapsulation of SC in alginate microcapsules, namely, "Medium size" (MSM) and "Coherent" Microcapsules (CM).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.