Many researchers have studied the biological effects of carotenoids and the more appropriate procedure for extracting them from vegetable sources. In this work we propose a rapid and low-cost procedure to extract lycopene from tomato in order to by-pass the problems related to the high cost of this molecule. Following this procedure we have obtained over 95% pure all-trans-lycopene checked by DAD-HPLC coupled with mass-spectrometer equipped with APCI source and by UV-Vis spectroscopy. Moreover, in order to evaluate the effectiveness of this procedure, we have assayed the capacity of the extracted lycopene to inhibit proliferation in T-lymphocyte jurkat J32 cells in comparison with authentic standard all-trans-lycopene. On this cellular line both standard lycopene and extracted lycopene tended to be dose-dependent but this latter seems to be more active even at lowest concentration.

Innovative extraction procedure for obtaining high pure lycopene from tomato

MONTESANO, Domenico;FALLARINO, Francesca;COSSIGNANI, Lina;SIMONETTI, Maria Stella;PUCCETTI, Paolo;DAMIANI, Pietro
2008

Abstract

Many researchers have studied the biological effects of carotenoids and the more appropriate procedure for extracting them from vegetable sources. In this work we propose a rapid and low-cost procedure to extract lycopene from tomato in order to by-pass the problems related to the high cost of this molecule. Following this procedure we have obtained over 95% pure all-trans-lycopene checked by DAD-HPLC coupled with mass-spectrometer equipped with APCI source and by UV-Vis spectroscopy. Moreover, in order to evaluate the effectiveness of this procedure, we have assayed the capacity of the extracted lycopene to inhibit proliferation in T-lymphocyte jurkat J32 cells in comparison with authentic standard all-trans-lycopene. On this cellular line both standard lycopene and extracted lycopene tended to be dose-dependent but this latter seems to be more active even at lowest concentration.
2008
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/152420
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