The aim of the present study was to investigate the impact of a probiotic product commercially distributed, consisting of a mix of four different species of Bifidobacterium [i.e. (B. bifidum, B. breve, B. longum, B. infantis) (Bifiselle)] , on the DNA-damaging effects of 2- amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) or acrylamide. Male CD1 mice were treated orally with PhIP or acrylamide. Suspensions of bifidobacteria were given by gavage to the animals 3 h before administration of model genotoxins. Subsequently, the extent of DNA migration was measured in colon and liver cells by the single-cell microgelelectrophoresis (comet) assay. The Bifidobacterium strains mix suspension caused a significant inhibition of DNA damage induced by PhIP in the colon and by acrylamide in the liver.
In vivo antigenotoxic properties of a commercial probiotic supplement containing bifidobacteria
DOMINICI, LUCA;MORETTI, Massimo;VILLARINI, Milena;VANNINI, SAMUELE;CENCI, Giovanni;TRAINA, Giovanna
2011
Abstract
The aim of the present study was to investigate the impact of a probiotic product commercially distributed, consisting of a mix of four different species of Bifidobacterium [i.e. (B. bifidum, B. breve, B. longum, B. infantis) (Bifiselle)] , on the DNA-damaging effects of 2- amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) or acrylamide. Male CD1 mice were treated orally with PhIP or acrylamide. Suspensions of bifidobacteria were given by gavage to the animals 3 h before administration of model genotoxins. Subsequently, the extent of DNA migration was measured in colon and liver cells by the single-cell microgelelectrophoresis (comet) assay. The Bifidobacterium strains mix suspension caused a significant inhibition of DNA damage induced by PhIP in the colon and by acrylamide in the liver.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
