Purpose. To evaluate a number of abrasive agents (calcium carbonate, calcium phosphate, sodium bicarbonate, and colloidal silica) on the in vitro and chew-out release of KSL (W) from a chewing gum formulation. Methods. The release of KSL (W) from the gum formulation was studied using a chewing apparatus (artificial saliva at 37°C, 50 strokes/min) and a chew-out method. KSL (W) was analyzed by reversed-phase HPLC. Results. Without an abrasive agent, approximately 30 and 75% of KSL (W) was released from the gum formulation after 10 and 40 minutes, respectively. The addition of calcium carbonate increased the release of the peptide by approximately 10%, while calcium phosphate did not show any substantial effect on the release of KSL (W). Colloidal silica did not affect the release while sodium bicarbonate decreased the release. In vivo chew-out studies indicated that the release of KSL (W) was higher than that obtained using the chewing machine apparatus. The taste and the consistency of gums containing the abrasive agents were acceptable with the exception of those containing colloidal silica. The silica particles aggregate between the teeth and irritate the gum tissues. Because of these observations, the colloidal silica does not appear to be a good candidate as an abrasive agent. Conclusion. The results suggest that calcium carbonate and calcium phosphate are suitable abrasive agents in this antibacterial/antiplaque gum formulation.

Evaluation of Abrasive Agents on the In Vitro and Chew-Out Release of a Decapeptide from a Chewing Gum Formulation

BLASI, PAOLO;
2007

Abstract

Purpose. To evaluate a number of abrasive agents (calcium carbonate, calcium phosphate, sodium bicarbonate, and colloidal silica) on the in vitro and chew-out release of KSL (W) from a chewing gum formulation. Methods. The release of KSL (W) from the gum formulation was studied using a chewing apparatus (artificial saliva at 37°C, 50 strokes/min) and a chew-out method. KSL (W) was analyzed by reversed-phase HPLC. Results. Without an abrasive agent, approximately 30 and 75% of KSL (W) was released from the gum formulation after 10 and 40 minutes, respectively. The addition of calcium carbonate increased the release of the peptide by approximately 10%, while calcium phosphate did not show any substantial effect on the release of KSL (W). Colloidal silica did not affect the release while sodium bicarbonate decreased the release. In vivo chew-out studies indicated that the release of KSL (W) was higher than that obtained using the chewing machine apparatus. The taste and the consistency of gums containing the abrasive agents were acceptable with the exception of those containing colloidal silica. The silica particles aggregate between the teeth and irritate the gum tissues. Because of these observations, the colloidal silica does not appear to be a good candidate as an abrasive agent. Conclusion. The results suggest that calcium carbonate and calcium phosphate are suitable abrasive agents in this antibacterial/antiplaque gum formulation.
2007
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/714512
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