A sensitive and precise high performance liquid chromatographic method for the simultaneous quantitation of S-adenosylmethionine SAMe), and S-adenosylhomocysteine (SAH) in rat liver is described. Liver is extracted by homogenization in 1.S M: PCA. SAMe and SAH are purified from contaminating substances by chromatography on Dowex 5O W x 2 and analyzed by HPLC on conventional styrene-type anion exchanger. Analytical chromatography, performed under isocratic elution conditions, is achieved in 18 min. SAH is determined as such while SAMe as adenine after hydrolysis at 60° C, under alkaline conditions. The sensitivity of the analytical procedure allows the determination of SAM and SAH in less than 200 mg of liver sample.

High performance liquid chromatographic analysis of S-adenosyl methionine and S-adenosyl homocysteine in the rat liver

FINI, Carlo;PALMERINI, Carlo Alberto;
1979

Abstract

A sensitive and precise high performance liquid chromatographic method for the simultaneous quantitation of S-adenosylmethionine SAMe), and S-adenosylhomocysteine (SAH) in rat liver is described. Liver is extracted by homogenization in 1.S M: PCA. SAMe and SAH are purified from contaminating substances by chromatography on Dowex 5O W x 2 and analyzed by HPLC on conventional styrene-type anion exchanger. Analytical chromatography, performed under isocratic elution conditions, is achieved in 18 min. SAH is determined as such while SAMe as adenine after hydrolysis at 60° C, under alkaline conditions. The sensitivity of the analytical procedure allows the determination of SAM and SAH in less than 200 mg of liver sample.
1979
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/916771
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