Recently CLA isomers have received considerable attention as potential anti-cancer agents. The aim of the study was to assess the genotoxicity/antigenotoxicity in vitro of linoleic acid (LA, c,c-C18:2, D-9), CLA isomer mixtures and homogeneous CLA TAGs (TriCLA) using the comet assay, to evaluate the effects on the extent of DNA injury in human hepatoma (HepG2) cells. The study was carried out both on commercial CLA (CLAc) and on CLA synthesized from grapestone oil (CLAg). The CLA isomer mixtures had different isomer profiles, determined by silver-ion HPLC (Agþ-HPLC), in particular CLAc was characterized by four main isomers (t8,c10; c9,t11; t10,c12; c11,t13), while CLAg showed two main isomers (c9,t11; t10,c12). As regards antigenotoxicity testing, LA, TriCLAg, and above all TriCLAc were effective antigenotoxic compounds against ethylmethanesulfonate (EMS) induced genotoxicity, while LA and CLAg were almost equally effective against 4-nitroquinoline N-oxide (4NQO) induced DNA damage. Both TriCLAc and TriCLAg showed an increased antigenotoxic activity toward EMS and a lower antigenotoxic activity toward 4NQO, with respect to both CLAc and CLAg. The higher capability of CLAg with respect to CLAc in counteracting the genotoxicity of 4NQO could be due to the different CLA isomer composition.

In vitro genotoxicity/antigenotoxicity testing of some conjugated linoleic acid isomers using comet assay

BLASI, FRANCESCA;DOMINICI, LUCA;MORETTI, Massimo;VILLARINI, Milena;MAURELLI, SILVIA;SIMONETTI, Maria Stella;DAMIANI, Pietro;COSSIGNANI, Lina
2012

Abstract

Recently CLA isomers have received considerable attention as potential anti-cancer agents. The aim of the study was to assess the genotoxicity/antigenotoxicity in vitro of linoleic acid (LA, c,c-C18:2, D-9), CLA isomer mixtures and homogeneous CLA TAGs (TriCLA) using the comet assay, to evaluate the effects on the extent of DNA injury in human hepatoma (HepG2) cells. The study was carried out both on commercial CLA (CLAc) and on CLA synthesized from grapestone oil (CLAg). The CLA isomer mixtures had different isomer profiles, determined by silver-ion HPLC (Agþ-HPLC), in particular CLAc was characterized by four main isomers (t8,c10; c9,t11; t10,c12; c11,t13), while CLAg showed two main isomers (c9,t11; t10,c12). As regards antigenotoxicity testing, LA, TriCLAg, and above all TriCLAc were effective antigenotoxic compounds against ethylmethanesulfonate (EMS) induced genotoxicity, while LA and CLAg were almost equally effective against 4-nitroquinoline N-oxide (4NQO) induced DNA damage. Both TriCLAc and TriCLAg showed an increased antigenotoxic activity toward EMS and a lower antigenotoxic activity toward 4NQO, with respect to both CLAc and CLAg. The higher capability of CLAg with respect to CLAc in counteracting the genotoxicity of 4NQO could be due to the different CLA isomer composition.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/922374
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