In the last decade proteomic has become powerful tool in determining functions of milk proteins, from nutritional and allergenic standpoint, and in monitoring health status of the mammary gland. However proteomic studies on ovine milk are still limited. The aims of the study was to identify, new protein biomarkers useful in the sanitary control and in the assessment of nutritional technological value of ewe milk. Milk Fat Globules (MFG) and whey milk, obtained from both healty and affected by Coagulase negative Staphylococci animals were analysed by using a proteomic approach based on 2-DE and combined mass spectrometry procedures. A comparison between mastitic and control samples showed a protein change due to a different molecular systems related to synthesis and transport of milk fat droplets, specific immunity in the mammary gland, structural and functional proteins correlated with chemical and physical properties of milk. These proteins, after validation could be considered as candidate biomarkers to monitoring mammary gland status, well-being of the sheep flock and milk quality.
Subclinical mastitis cause to coagulase negative staphylococci in ewe: milk proteomic analysis
CHIARADIA, Elisabetta;TARTAGLIA, MICAELA;SCOPPETTA, FAUSTO;AVELLINI, Luca;
2012
Abstract
In the last decade proteomic has become powerful tool in determining functions of milk proteins, from nutritional and allergenic standpoint, and in monitoring health status of the mammary gland. However proteomic studies on ovine milk are still limited. The aims of the study was to identify, new protein biomarkers useful in the sanitary control and in the assessment of nutritional technological value of ewe milk. Milk Fat Globules (MFG) and whey milk, obtained from both healty and affected by Coagulase negative Staphylococci animals were analysed by using a proteomic approach based on 2-DE and combined mass spectrometry procedures. A comparison between mastitic and control samples showed a protein change due to a different molecular systems related to synthesis and transport of milk fat droplets, specific immunity in the mammary gland, structural and functional proteins correlated with chemical and physical properties of milk. These proteins, after validation could be considered as candidate biomarkers to monitoring mammary gland status, well-being of the sheep flock and milk quality.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
