Many techniques are currently available for the ex situ conservation of valuable germplasm. The in vitro cultures, with particolar reference to the micropropagation, are progressively used in order to safeguard the plant biodiversity. However, the recurrent repetiton of subcultures could induce adaptation to heterotrophic conditions or genetic instability. To this end, a first experiment was conducted with the aim to assess a mixed system based on the alternation of the storage at low temperatures with the proliferation subculture of vitroderived explants of a venerable pear tree („San Bartolomeo‟) discovered in Umbria region (within the district of Gubbio). In addition, a second experiment was carried out with the goal to evaluate the use of the encapsulation technology in order to propose an effective tool for plant germplasm conservation and for a simplified exchange system between laboratories. The preliminary results showed the suitability of the tested procedure, consisting in 3 months storage at 4°C followed by a conventional subculture into the growth chamber. In fact, a satisfactory rate of viability (65%) and regrowth (63,3%) of the „San Bartolomeo‟ explants were recorded (Experiment 1). In order to the second experiment the encapsulated microcuttings (capsules) showed high viability (85%) and a reasonable regrowth (55%), after 8 weeks of storage at low temperatures, in evidence of the effectiveness of the encapsulation technology also for this genotype.
Prime valutazioni sulla frigoconservazione di propaguli vitro-derivati di un’antica varietà frutticola umbra: il Pero di San Bartolomeo
MICHELI, Maurizio;GARDI, Tiziano;PROSPERI, Francesco;SISANI, Giorgio;
2013
Abstract
Many techniques are currently available for the ex situ conservation of valuable germplasm. The in vitro cultures, with particolar reference to the micropropagation, are progressively used in order to safeguard the plant biodiversity. However, the recurrent repetiton of subcultures could induce adaptation to heterotrophic conditions or genetic instability. To this end, a first experiment was conducted with the aim to assess a mixed system based on the alternation of the storage at low temperatures with the proliferation subculture of vitroderived explants of a venerable pear tree („San Bartolomeo‟) discovered in Umbria region (within the district of Gubbio). In addition, a second experiment was carried out with the goal to evaluate the use of the encapsulation technology in order to propose an effective tool for plant germplasm conservation and for a simplified exchange system between laboratories. The preliminary results showed the suitability of the tested procedure, consisting in 3 months storage at 4°C followed by a conventional subculture into the growth chamber. In fact, a satisfactory rate of viability (65%) and regrowth (63,3%) of the „San Bartolomeo‟ explants were recorded (Experiment 1). In order to the second experiment the encapsulated microcuttings (capsules) showed high viability (85%) and a reasonable regrowth (55%), after 8 weeks of storage at low temperatures, in evidence of the effectiveness of the encapsulation technology also for this genotype.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
