The aim of the present study was to evaluate the ability of PhSeZnCl to induce cytotoxicity and/or genetic damage in vitro. Human hepatoma cells, HepG2, were exposed to increasing concentrations of PhSeZnCl (from 10 to 0,1 μM) for 4 h at 37ºC. Cytotoxicity was assessed either by acridine orange/DAPI staining and trypan blue exclusion test. Non cytotoxic doses were further analysed for genotoxicity by means of the single cell microgel (comet) assay. The results showed that exposure of HepG2 cells to the given compound resulted in a significant cytotoxic effect up to a concentrations of 2 μM. With respect to genotoxicity, only the highest non cytotoxic concentrations of PhSeZnCl (1 μM) produced significant genetic damage, with a high proportion of apoptotic cell. Since apoptosis induced by DNA damage is an important mechanism of tumor suppression, further studies are required to clarify the genotoxic and apoptotis-inducing effects of PhSeZnCl.
Investigation of in vitro cytotoxicity and genotoxicity of a new organoselenium compound: PhSeZnCl
LEVORATO, SARA;MORETTI, Massimo;SANTI, Claudio
2013
Abstract
The aim of the present study was to evaluate the ability of PhSeZnCl to induce cytotoxicity and/or genetic damage in vitro. Human hepatoma cells, HepG2, were exposed to increasing concentrations of PhSeZnCl (from 10 to 0,1 μM) for 4 h at 37ºC. Cytotoxicity was assessed either by acridine orange/DAPI staining and trypan blue exclusion test. Non cytotoxic doses were further analysed for genotoxicity by means of the single cell microgel (comet) assay. The results showed that exposure of HepG2 cells to the given compound resulted in a significant cytotoxic effect up to a concentrations of 2 μM. With respect to genotoxicity, only the highest non cytotoxic concentrations of PhSeZnCl (1 μM) produced significant genetic damage, with a high proportion of apoptotic cell. Since apoptosis induced by DNA damage is an important mechanism of tumor suppression, further studies are required to clarify the genotoxic and apoptotis-inducing effects of PhSeZnCl.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
