In the vineyard, the control of fungal pathogens is a key step to obtain high quality grapes. The Integrated Pest Management, mandatory in the European Community, along with the market demanding products from organic or biodynamic agriculture, places limits to the use of synthetic pesticides. Effective means alternative to chemicals are thus needed. Extracts from seaweeds are used in agriculture as biostimulants, due their ability to promote plant growth and increase yield. Furthermore, they can protect plants against biotic and abiotic stress. Extracts act directly against plant pathogens through their antimicrobial compounds and/or indirectly, increasing host resistance by means of their polysaccharides and other compounds. Here, Ascophyllus nodosum extract (Acadian Seaweed Marine Dry Powder, Biogard, Italy; 1.5 kg ha−1) was sprayed on 3-years-old potted vines of cv. Sangiovese six times during the season 2015, from 3 June with weekly intervals, and berries were sampled at 0 and 24 h after the last treatment (hpt). Eva Green-based qPCR performed on defense-related genes showed a significant accumulation of the VvPR1 (Pathogenesis-related protein 1) and VvCaS2 (Callose Synthase 2) genes in berries harvested 24 hpt from treated vines, with respect to the untreated ones. At the same time, not significant differences were observed between treated and untreated samples for the VvLOX (lipoxygenase). In ripe berries detached from treated vines and artificially inoculated with Botrytis cinerea, the Index Percentage of Infection was significantly lower respect to the control. Our results support the role of A. nodosum extract in plant defense.

The seaweeds Ascophyllum nodosum commercial extract induces resistance in grapevine and protect against Botrytis cinerea

QUAGLIA, Mara;MORETTI, Chiaraluce;FRIONI, TOMMASO;TOMBESI, SERGIO;PALLIOTTI, Alberto
2016

Abstract

In the vineyard, the control of fungal pathogens is a key step to obtain high quality grapes. The Integrated Pest Management, mandatory in the European Community, along with the market demanding products from organic or biodynamic agriculture, places limits to the use of synthetic pesticides. Effective means alternative to chemicals are thus needed. Extracts from seaweeds are used in agriculture as biostimulants, due their ability to promote plant growth and increase yield. Furthermore, they can protect plants against biotic and abiotic stress. Extracts act directly against plant pathogens through their antimicrobial compounds and/or indirectly, increasing host resistance by means of their polysaccharides and other compounds. Here, Ascophyllus nodosum extract (Acadian Seaweed Marine Dry Powder, Biogard, Italy; 1.5 kg ha−1) was sprayed on 3-years-old potted vines of cv. Sangiovese six times during the season 2015, from 3 June with weekly intervals, and berries were sampled at 0 and 24 h after the last treatment (hpt). Eva Green-based qPCR performed on defense-related genes showed a significant accumulation of the VvPR1 (Pathogenesis-related protein 1) and VvCaS2 (Callose Synthase 2) genes in berries harvested 24 hpt from treated vines, with respect to the untreated ones. At the same time, not significant differences were observed between treated and untreated samples for the VvLOX (lipoxygenase). In ripe berries detached from treated vines and artificially inoculated with Botrytis cinerea, the Index Percentage of Infection was significantly lower respect to the control. Our results support the role of A. nodosum extract in plant defense.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11391/1413795
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