In the present study, we evaluated the expression of nerve growth factor (NGF) and cognate receptors, the neurotrophic tyrosine kinase receptor 1 (NTRK1) and nerve growth factor receptor (NGFR) in the anterior pituitary, ovary, and cervix of rabbits. Positive immuno-signals for NGF, NTRK1, and NGFR were detected in all tissues. RT-PCR confirmed the presence of mRNA for NGF, NTRK1, and NGFR in the same tissues, but not that of NTRK1 in the cervix. Present data confirm that seminal plasma NGF may induce ovulation in rabbits via direct endocrine mechanisms through binding to cognate receptors localized in the pituitary and/or ovary following adsorption from uterine/cervical mucosa.
Expression of nerve growth factor and its receptors in the pituitary and reproductive tract of rabbits (Oryctolagus cuniculus): possible NGF-mediated role to induce ovulation
M Maranesi
;L PetrucciMembro del Collaboration Group
;L LeonardiMembro del Collaboration Group
;F PiroMembro del Collaboration Group
;F ParilloMembro del Collaboration Group
;M ZeraniMembro del Collaboration Group
;C BoitiMembro del Collaboration Group
2017
Abstract
In the present study, we evaluated the expression of nerve growth factor (NGF) and cognate receptors, the neurotrophic tyrosine kinase receptor 1 (NTRK1) and nerve growth factor receptor (NGFR) in the anterior pituitary, ovary, and cervix of rabbits. Positive immuno-signals for NGF, NTRK1, and NGFR were detected in all tissues. RT-PCR confirmed the presence of mRNA for NGF, NTRK1, and NGFR in the same tissues, but not that of NTRK1 in the cervix. Present data confirm that seminal plasma NGF may induce ovulation in rabbits via direct endocrine mechanisms through binding to cognate receptors localized in the pituitary and/or ovary following adsorption from uterine/cervical mucosa.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
