The ability of nitric oxide releasing compounds, sodium nitroprusside (SNP) and S-nitrosocysteine (CysNO), to induce resistance in Arabidopsis thaliana plants (Col-0) against tobacco necrosis virus (TNV) was evaluated. SNP and CysNO were able to systemically and significantly (P≤0.05) protect Arabidopsis plants against TNV, with protection levels of 63 and 71%, respectively. A protection level of 66% was obtained with the SAR inducer acibenzolar-S-methyl as comparison. To verify whether the protection exerted by SNP and CysNO is mediated by salicylic acid, transgenic A. thaliana plants expressing the nahG gene were used. Reduced protection levels (38%) were observed in NahG Arabidopsis plants treated with SNP or CysNO in comparison with the wild-type plants. It is noteworthy that untreated NahG plants are less susceptible to TNV infection than wild-type plants. Our results suggest that the protection induced by the nitric oxide releasing compounds used are in part mediated by salicylic acid.

Nitric oxide releasing compounds systemically protect Arabidopsis thaliana plants from Tobacco Mosaic

MORETTI, Chiaraluce;SILVESTRI, SIMONA;BUONAURIO, Roberto
2006

Abstract

The ability of nitric oxide releasing compounds, sodium nitroprusside (SNP) and S-nitrosocysteine (CysNO), to induce resistance in Arabidopsis thaliana plants (Col-0) against tobacco necrosis virus (TNV) was evaluated. SNP and CysNO were able to systemically and significantly (P≤0.05) protect Arabidopsis plants against TNV, with protection levels of 63 and 71%, respectively. A protection level of 66% was obtained with the SAR inducer acibenzolar-S-methyl as comparison. To verify whether the protection exerted by SNP and CysNO is mediated by salicylic acid, transgenic A. thaliana plants expressing the nahG gene were used. Reduced protection levels (38%) were observed in NahG Arabidopsis plants treated with SNP or CysNO in comparison with the wild-type plants. It is noteworthy that untreated NahG plants are less susceptible to TNV infection than wild-type plants. Our results suggest that the protection induced by the nitric oxide releasing compounds used are in part mediated by salicylic acid.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/11391/33317
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