Giant cell tumor of bone (GCTB) is generally an intramedullary tumor with variable and unpredictable potential for growth. GCTB is a neoplasm formed by a network of spindle-shaped mononuclear stromal cells and multinuclear giant cells similar to osteoclasts. The cellular components interact together with various factors playing also a role in osteoclast function regulation as receptor activator of nuclear factor kappa-B ligand (RANKL), receptor activator of nuclear factor kappa-B (RANK), and osteoprotegerin (OPG) as the key molecular regulation system for bone remodelling. RANKL is the main stimulatory factor for formation of mature osteoclasts and is essential for their survival. Precursors of osteoclasts express the receptor activator nuclear factor kB, RANK, and in presence of macrophage colony-stimulating factor (M-CSF) and its ligand, RANKL, it mediates osteoclast formation by increasing the expression of enzymes that dissolve organic and inorganic components of bone, such as MMP-9, uPA proteolytic system. So, these interactions may provide information to develop new approaches for a biological therapy of this tumor. Drugs that target the osteolitic process lower recurrence rate associated to morbidity and mortality and are considered useful for new clinical treatments. The aim of this study was to compare the potential biomarkers involved in bone remodeling and cell proliferation in human and animals GCTB , in the attempt to define possible common activated pathways or key molecules in the vicious cycle of osteolytic and proliferative process.
A COMPARATIVE STUDY ON PHENOTYPICAL AND BIOMOLECULAR CHARACTERIZATION OF GIANT CELL TUMOR OF BONE IN FELINE AND HUMAN SPECIES.
LEONARDI, Leonardo;MECHELLI, Luca;BELLEZZA, Enrico;
2012
Abstract
Giant cell tumor of bone (GCTB) is generally an intramedullary tumor with variable and unpredictable potential for growth. GCTB is a neoplasm formed by a network of spindle-shaped mononuclear stromal cells and multinuclear giant cells similar to osteoclasts. The cellular components interact together with various factors playing also a role in osteoclast function regulation as receptor activator of nuclear factor kappa-B ligand (RANKL), receptor activator of nuclear factor kappa-B (RANK), and osteoprotegerin (OPG) as the key molecular regulation system for bone remodelling. RANKL is the main stimulatory factor for formation of mature osteoclasts and is essential for their survival. Precursors of osteoclasts express the receptor activator nuclear factor kB, RANK, and in presence of macrophage colony-stimulating factor (M-CSF) and its ligand, RANKL, it mediates osteoclast formation by increasing the expression of enzymes that dissolve organic and inorganic components of bone, such as MMP-9, uPA proteolytic system. So, these interactions may provide information to develop new approaches for a biological therapy of this tumor. Drugs that target the osteolitic process lower recurrence rate associated to morbidity and mortality and are considered useful for new clinical treatments. The aim of this study was to compare the potential biomarkers involved in bone remodeling and cell proliferation in human and animals GCTB , in the attempt to define possible common activated pathways or key molecules in the vicious cycle of osteolytic and proliferative process.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
