evaluate the possibility to engineer skin on the chicken chorioallantoic membrane (CAM) starting from de-cellularized de-epidermized human dermis (DED) and immortalized keratinocytes with the aim of replacing animals in short-term investigations in dermatology. Our results indicate that it is possible to use the revascularized DED to support the formation of an epidermis provided with stratum corneum. The future goal of this project is to establish the epidermal barrier using primary epidermal cells and test the barrier function by examining the permeability to various chemical compounds.
On the possibility to bioengineer skin on chick chorioallantois
BLASI, PAOLO;BARBERINI, Lanfranco;ALBERTI, Paolo Francesco;CIROTTO, Carlo
2013
Abstract
evaluate the possibility to engineer skin on the chicken chorioallantoic membrane (CAM) starting from de-cellularized de-epidermized human dermis (DED) and immortalized keratinocytes with the aim of replacing animals in short-term investigations in dermatology. Our results indicate that it is possible to use the revascularized DED to support the formation of an epidermis provided with stratum corneum. The future goal of this project is to establish the epidermal barrier using primary epidermal cells and test the barrier function by examining the permeability to various chemical compounds.File in questo prodotto:
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