The aim of this study was to investigate the flowering variation and the molecular polymorphism in key regulatory genes that control flowering in a Phaseolus vulgaris L. collection of 94 accessions from Europe and the Americas. The analysis of variance revealed that the difference in days-to-flowering between accessions was significant, with European accessions characterized by flowering precocity. Population structure analysis corroborated previous data on the genetic distinction between the Andean and Mesoamerican gene pools. A low level of admixture was detected. Genomic sequences of 15 genes were produced, about 7.0 kb per accession were sequenced and a total of 48 nucleotide substitutions were identified. A Mixed Linear Model analysis, including population structure and kinship was used to identify marker-trait associations. Haplotype tagging single nucleotide polymorphisms (htSNPs) associated with the studied traits were detected: in PvVRN1 and PvPHYB with days-to-flowering, in PvMYB29 with number of flower buds per inflorescence and in PvTFL1z and PvFCA with inflorescence length. Two major pathways leading to flowering time control, photoperiod and vernalization pathways were found among genes that displayed significant associations. In particular, the PvVRN1 gene appears to play an important role in regulating the adaptation process of common bean.
Molecular polymorphism related to flowering trait variation in a Phaseolus vulgaris L. collection
RAGGI, LORENZO;NEGRI, Valeria
2014
Abstract
The aim of this study was to investigate the flowering variation and the molecular polymorphism in key regulatory genes that control flowering in a Phaseolus vulgaris L. collection of 94 accessions from Europe and the Americas. The analysis of variance revealed that the difference in days-to-flowering between accessions was significant, with European accessions characterized by flowering precocity. Population structure analysis corroborated previous data on the genetic distinction between the Andean and Mesoamerican gene pools. A low level of admixture was detected. Genomic sequences of 15 genes were produced, about 7.0 kb per accession were sequenced and a total of 48 nucleotide substitutions were identified. A Mixed Linear Model analysis, including population structure and kinship was used to identify marker-trait associations. Haplotype tagging single nucleotide polymorphisms (htSNPs) associated with the studied traits were detected: in PvVRN1 and PvPHYB with days-to-flowering, in PvMYB29 with number of flower buds per inflorescence and in PvTFL1z and PvFCA with inflorescence length. Two major pathways leading to flowering time control, photoperiod and vernalization pathways were found among genes that displayed significant associations. In particular, the PvVRN1 gene appears to play an important role in regulating the adaptation process of common bean.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
