tHistopathological lesions caused by plerocercoids of Triaenophorus nodulosus within theliver of perch, Perca fluviatilis, from Lake Trasimeno were studied. Livers harbored 1–3 par-asite larvae and pathological alterations were more marked in those with 3 plerocercoids.In the liver, larvae were encysted, surrounded by a capsule of host tissue; two of 14 plero-cercoids were necrotic. In infected livers, some hepatocytes showed degenerative changes,i.e. swelling and hydropic degeneration, notably those in close proximity to larvae. By com-parison, hepatocytes in uninfected livers or in regions away from the point of infectionappeared normal. The occurrence of macrophage aggregates (MAs) distributed among themast cells (MCs) was observed around the encysted larvae. The cellular elements involvedin the immune response within liver were assessed by immunohistochemical techniquesand by the use of antibodies against the antimicrobial peptides piscidins 3 and 4, whichrevealed a sub-population of positive MCs. In infected livers, numerous MCs that wereimmunopositive to P4 and a few that were positive to P3 were found around T. nodulosuslarvae. Histological sections of both uninfected and infected liver were immunostained withproliferative cell nuclear antigen (PCNA) antibody. Within the capsule and in close proxim-ity to the parasite larvae, various cell types (i.e., MCs, fibroblasts and epithelioid cells) anda significantly higher number of PCNA-positive hepatocytes that were immunoreactive toPCNA were found compared to uninfected livers (ANOVA, P < 0.05). No parasites of any typewere found in gill, spleen, kidney or gonad of P. fluviatilis and the intestine of 3 perch wereinfected with few specimens of Acanthocephalus lucii.

Perch liver reaction to Triaenophorus nodulosus plerocercoids with an emphasis on piscidins 3, 4 and proliferative cell nuclear antigen (PCNA) expression

LORENZONI, Massimo;
2014

Abstract

tHistopathological lesions caused by plerocercoids of Triaenophorus nodulosus within theliver of perch, Perca fluviatilis, from Lake Trasimeno were studied. Livers harbored 1–3 par-asite larvae and pathological alterations were more marked in those with 3 plerocercoids.In the liver, larvae were encysted, surrounded by a capsule of host tissue; two of 14 plero-cercoids were necrotic. In infected livers, some hepatocytes showed degenerative changes,i.e. swelling and hydropic degeneration, notably those in close proximity to larvae. By com-parison, hepatocytes in uninfected livers or in regions away from the point of infectionappeared normal. The occurrence of macrophage aggregates (MAs) distributed among themast cells (MCs) was observed around the encysted larvae. The cellular elements involvedin the immune response within liver were assessed by immunohistochemical techniquesand by the use of antibodies against the antimicrobial peptides piscidins 3 and 4, whichrevealed a sub-population of positive MCs. In infected livers, numerous MCs that wereimmunopositive to P4 and a few that were positive to P3 were found around T. nodulosuslarvae. Histological sections of both uninfected and infected liver were immunostained withproliferative cell nuclear antigen (PCNA) antibody. Within the capsule and in close proxim-ity to the parasite larvae, various cell types (i.e., MCs, fibroblasts and epithelioid cells) anda significantly higher number of PCNA-positive hepatocytes that were immunoreactive toPCNA were found compared to uninfected livers (ANOVA, P < 0.05). No parasites of any typewere found in gill, spleen, kidney or gonad of P. fluviatilis and the intestine of 3 perch wereinfected with few specimens of Acanthocephalus lucii.
2014
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/1176677
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