Although the great majority of bacteria found in nature are living in multispecies communities, microbiological studies have historically focused on single species or competition and antagonism experiments between different species. Future directions need to focus much more on microbial communities in order to better understand what is happening in the wild. We are using the olive-knot disease as a model to study 25 the role and interaction of multispecies bacterial communities in disease establishment/development. In the olive-knot, non-pathogenic bacterial species (eg. Erwinia toletana) are coexisting with the pathogen (Pseudomonas savastanoi pv. savastanoi); we have demonstrated cooperation among these two species via quorum sensing signal sharing. The outcome of this interaction is a more aggressive disease 30 when co-inoculations are made compared to single ones. In planta experiments show that these two species are co-localizing in the olive-knot and this close proximity most probably facilitates exchange of quorum sensing signals and metabolites. In silico recreation of their metabolic pathways showed that they could have complementing pathways also implicating sharing of metabolites. Our microbiome studies of nine olive-knot samples have shown that the olive-knot community possesses great bacterial diversity, however the presence of five genera (ie. Pseudomonas, Pantoea, Curtobacterium, Pectobacterium and Erwinia) can be found in almost all samples.

Bacterial multispecies studies and microbiome analysis of a plant disease

MORETTI, Chiaraluce;BUONAURIO, Roberto;
2014

Abstract

Although the great majority of bacteria found in nature are living in multispecies communities, microbiological studies have historically focused on single species or competition and antagonism experiments between different species. Future directions need to focus much more on microbial communities in order to better understand what is happening in the wild. We are using the olive-knot disease as a model to study 25 the role and interaction of multispecies bacterial communities in disease establishment/development. In the olive-knot, non-pathogenic bacterial species (eg. Erwinia toletana) are coexisting with the pathogen (Pseudomonas savastanoi pv. savastanoi); we have demonstrated cooperation among these two species via quorum sensing signal sharing. The outcome of this interaction is a more aggressive disease 30 when co-inoculations are made compared to single ones. In planta experiments show that these two species are co-localizing in the olive-knot and this close proximity most probably facilitates exchange of quorum sensing signals and metabolites. In silico recreation of their metabolic pathways showed that they could have complementing pathways also implicating sharing of metabolites. Our microbiome studies of nine olive-knot samples have shown that the olive-knot community possesses great bacterial diversity, however the presence of five genera (ie. Pseudomonas, Pantoea, Curtobacterium, Pectobacterium and Erwinia) can be found in almost all samples.
2014
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/1192479
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