OBJECTIVE - To investigate concentration of plasma insulin glargine after its subcutaneous dosing comparedwith concentration of its metabolites 1 (M1) and 2 (M2) in subjectswith type 2 diabetes. RESEARCH DESIGN AND METHODS - Nine subjects underwent a 32-h euglycemic glucose clamp study (0.4 units/kg glargine after 1 week of daily glargine administration). Glargine, M1, and M2 were measured by a specific liquid chromatography-tandem mass spectrometry assay. RESULTS - Glargine was detected in only five of the nine subjects, at few time points, and at negligible concentrations. M1 was detected in all subjects and exhibited the same pattern as traditional radioimmunoassay-measured plasma insulin. M2 was not detected at all. CONCLUSIONS - After subcutaneous injection, glargine was minimally detectable in blood, whereas its metabolite M1 accounted for most (>90%) of the plasma insulin concentration and metabolic action of the injected glargine
Metabolism of insulin glargine after repeated daily subcutaneous injections in subjects with type 2 diabetes.
LUCIDI, Paola;PORCELLATI, Francesca;ROSSETTI, Paolo;CANDELORO, Paola;CIOLI, PATRIZIA;BOLLI, Geremia Brunetto;FANELLI, Carmine Giuseppe
2012
Abstract
OBJECTIVE - To investigate concentration of plasma insulin glargine after its subcutaneous dosing comparedwith concentration of its metabolites 1 (M1) and 2 (M2) in subjectswith type 2 diabetes. RESEARCH DESIGN AND METHODS - Nine subjects underwent a 32-h euglycemic glucose clamp study (0.4 units/kg glargine after 1 week of daily glargine administration). Glargine, M1, and M2 were measured by a specific liquid chromatography-tandem mass spectrometry assay. RESULTS - Glargine was detected in only five of the nine subjects, at few time points, and at negligible concentrations. M1 was detected in all subjects and exhibited the same pattern as traditional radioimmunoassay-measured plasma insulin. M2 was not detected at all. CONCLUSIONS - After subcutaneous injection, glargine was minimally detectable in blood, whereas its metabolite M1 accounted for most (>90%) of the plasma insulin concentration and metabolic action of the injected glargineI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
