Sheep buccal glands consist of mucous acini capped by demilunes. Information on the chemical structure of their secretory glycoconjugates were obtained by means of a battery of peroxidase conjugated lectins with affinity for specific terminal and/or internal sugars. Neuraminidase procedures followed by lectin staining were also used to visualize the carbohydrate sequence. Stored secretions lit mucous acinar cells contained glycoconjugates with N-acetylglucosamine and sialic acid linked to alpha N-acetylgalactosamine and galactosyl (beta 1 -> 3) N-acetylgalactosamine. Demilunar cells displayed fucose, mannose, N-acetylglucosamine and alpha,Nacetylgalactosamine residues. Cells lining duct system showed a very strong staining at the apical surface and in the cytoplasm with UEA I, LTA and ConA.
GLYCOCONJUGATES IN SHEEP BUCCAL GLANDS INVESTIGATED BY CONVENTIONAL AND LECTIN HISTOCHEMISTRY
PEDINI, Vera
;DALL'AGLIO, Cecilia;MERCATI, FRANCESCA;PASCUCCI, Luisa;
2008
Abstract
Sheep buccal glands consist of mucous acini capped by demilunes. Information on the chemical structure of their secretory glycoconjugates were obtained by means of a battery of peroxidase conjugated lectins with affinity for specific terminal and/or internal sugars. Neuraminidase procedures followed by lectin staining were also used to visualize the carbohydrate sequence. Stored secretions lit mucous acinar cells contained glycoconjugates with N-acetylglucosamine and sialic acid linked to alpha N-acetylgalactosamine and galactosyl (beta 1 -> 3) N-acetylgalactosamine. Demilunar cells displayed fucose, mannose, N-acetylglucosamine and alpha,Nacetylgalactosamine residues. Cells lining duct system showed a very strong staining at the apical surface and in the cytoplasm with UEA I, LTA and ConA.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
