The purpose of this research was to assess the physicochemical properties of a controlled release formulation of recombinant human growth hormone (rHGH) encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) composite microspheres. rHGH was loaded in poly(acryloyl hydroxyethyl) starch (acHES) microparticles, and then the protein-containing microparticles were encapsulated in the PLGA matrix by a solvent extraction/evaporation method. rHGH-loaded PLGA microspheres were also prepared using mannitol without the starch hydrogel microparticle microspheres for comparison.  The detection of secondary structure changes in protein was investigated by using a Fourier Transfer Infrared (FTIR) technique.  The composite microspheres were spherical in shape (44.6 +/- 2.47 microm), and the PLGA-mannitol microspheres were 39.7 +/- 2.50 microm.  Drug-loading efficiency varied from 93.2% to 104%.  The composite microspheres showed higher overall drug release than the PLGA/mannitol microspheres.  FTIR analyses indicated good stability and structural integrity of HGH localized in the microspheres.  The PLGA-acHES composite microsphere system could be useful for the controlled delivery of protein drugs.

Preparation and characterization of poly(D,L-lactide-co-glycolide) microspheres for controlled release of human growth hormone

GIOVAGNOLI, Stefano;
2003

Abstract

The purpose of this research was to assess the physicochemical properties of a controlled release formulation of recombinant human growth hormone (rHGH) encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) composite microspheres. rHGH was loaded in poly(acryloyl hydroxyethyl) starch (acHES) microparticles, and then the protein-containing microparticles were encapsulated in the PLGA matrix by a solvent extraction/evaporation method. rHGH-loaded PLGA microspheres were also prepared using mannitol without the starch hydrogel microparticle microspheres for comparison.  The detection of secondary structure changes in protein was investigated by using a Fourier Transfer Infrared (FTIR) technique.  The composite microspheres were spherical in shape (44.6 +/- 2.47 microm), and the PLGA-mannitol microspheres were 39.7 +/- 2.50 microm.  Drug-loading efficiency varied from 93.2% to 104%.  The composite microspheres showed higher overall drug release than the PLGA/mannitol microspheres.  FTIR analyses indicated good stability and structural integrity of HGH localized in the microspheres.  The PLGA-acHES composite microsphere system could be useful for the controlled delivery of protein drugs.
2003
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/124807
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