The Marchigiana is famous for its large body size and favourable dressing percentage. A myostatin (MSTN) gene mutation was identified in Marchigiana, a G to T transversion. The homozygote “GG” yields a “normal” phenotype, the homozygote “TT” yields a double muscled body shape but sometimes causes survival problems, and the heterozygote genotype produces an extremely muscled body without defects. In practice, Marchigiana “TT” homozygotes are culled from reproduction, but the heterozygotes are chosen as sires. The objective of this study was to assess genes involved in Marchigiana muscle development to improve selection procedures. The effects of the MSTN and myogenic factor 5 (MYF5) genes on the growth and muscle traits in the Marchigiana breed were assessed. The effects of MSTN together with the genotype of the causative mutation (g.874G>T) and the effects of the two SNPs in the promoter were studied (g.-371T>A, g.-805G>C). The SNP effects were evaluated in a comparison between the means of the several genotypes or for the average gene substitution and dominance effect. Two hundred and forty-nine bullocks were evaluated using a performance test. At the beginning and end of the trial, the animals were weighed and their bodies were measured every 21 days up to 12 months of age. In addition to these observations, morphological scores and the BLUP indexes were estimated at the end of the performance test. The obtained results suggested that the MSTN g.874G>T and MYF5 SNPs could be considered in the selection program of the Marchigiana breed. A MSTN g.874G>T genotyping service for the breeders could help to avoid the “TT” genotype and to select for the “GT” genotype. The “AA” MYF5 SNP genotype could also be selected for even if good muscle development yields a certain size reduction.
Influence of single nucleotide polymorphisms in the myostatin and myogenic factor 5 muscle growth-related genes on the performance traits of Marchigiana beef cattle
SARTI, Francesca Maria;LASAGNA, Emiliano;CECCOBELLI, Simone;DI LORENZO, PIERA;GIONTELLA, ANDREA;PIERAMATI, Camillo;PANELLA, Francesco
2014
Abstract
The Marchigiana is famous for its large body size and favourable dressing percentage. A myostatin (MSTN) gene mutation was identified in Marchigiana, a G to T transversion. The homozygote “GG” yields a “normal” phenotype, the homozygote “TT” yields a double muscled body shape but sometimes causes survival problems, and the heterozygote genotype produces an extremely muscled body without defects. In practice, Marchigiana “TT” homozygotes are culled from reproduction, but the heterozygotes are chosen as sires. The objective of this study was to assess genes involved in Marchigiana muscle development to improve selection procedures. The effects of the MSTN and myogenic factor 5 (MYF5) genes on the growth and muscle traits in the Marchigiana breed were assessed. The effects of MSTN together with the genotype of the causative mutation (g.874G>T) and the effects of the two SNPs in the promoter were studied (g.-371T>A, g.-805G>C). The SNP effects were evaluated in a comparison between the means of the several genotypes or for the average gene substitution and dominance effect. Two hundred and forty-nine bullocks were evaluated using a performance test. At the beginning and end of the trial, the animals were weighed and their bodies were measured every 21 days up to 12 months of age. In addition to these observations, morphological scores and the BLUP indexes were estimated at the end of the performance test. The obtained results suggested that the MSTN g.874G>T and MYF5 SNPs could be considered in the selection program of the Marchigiana breed. A MSTN g.874G>T genotyping service for the breeders could help to avoid the “TT” genotype and to select for the “GT” genotype. The “AA” MYF5 SNP genotype could also be selected for even if good muscle development yields a certain size reduction.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
