Background and studv aim: Spontaneous chromosomal instability has been correlated with a risk of developing cancer. We evaluated sister chromatid exchange (SCE) and micronuclei (MN) frequency in peripheral blood lymphocytes of early-stage breast cancer patients to see if it can be considered a disease biomarker. Materials and Methods; In 20 evaluable pts, aged 38 to 81 years (median 57.5), SCE and MN were measured both before and four weeks after conservative surgery. While, in those 10 pts who had previously received chemotherapy (CT), testing was done immediately before radiotherapy (RT). Further Controls were done 8 weeks after RT on all pts and at 6 months in 9 cases. All pts will be studied regularly during follow-up. There were 15 ductal infiltrant carcinomas (13 G2, 2 G3), 1 medullary carcinoma, 1 adenocarcinoma and 3 intraductal carcinomas with microinfiltration. Median tumor size was 12 mm (range 7-27 mm). In 16 and 11 cases estrogen and progestinic receptors were positive, respectively. Five pts had axillary positive nodes and in three of these cases they were four or more. Ten pts underwent adjuvant chemotherapy (CMF or FEC). Honnonotherapy was prescribed to 14 cases. RT was delivered to thè breast ± sovraclavicular nodes; single dose was 1.8-2 Gy, total dose 50.4-50 Gy; a 10 Gy boost was delivered to thè tumor bed. Student’s t test compared SCE and MN basai values to both those from a healthy control group of 7 women and those values obtained from treatment/follow-up times. Results:SCE value reduction after surgery, though not statistically significant, (p 0.07) seems to be a result of tumor removai while thè SCE increase after chemotherapy (p 0.04) is most likely to be a result of cytotoxic damage. MN increase after RT (p < 0.01) is most likely due to genotoxic damage. A statistically significant difference (p 0.04) was observed between SCE basai and control group values. Conclusions: The frequency of SCE as a cancer biomarker was confirmed by thè difference obtained comparing basai testing to control group values. Results here suggest that SCE and MN must be an index of damage due to CT and RT, respectively. In thè future, this study will seek to determine if SCE and MN frequency measurements during follow-up are disease progression predictors.
Sister chromatid exchange and micronuclei frequency in early-stage breast cancer patients: preliminary results of a prospective observational study.
ARISTEI, Cynthia;GUERRIERI, Paola;ARMELLINI PISANI, Rossana;RULLI, Antonio;
2004
Abstract
Background and studv aim: Spontaneous chromosomal instability has been correlated with a risk of developing cancer. We evaluated sister chromatid exchange (SCE) and micronuclei (MN) frequency in peripheral blood lymphocytes of early-stage breast cancer patients to see if it can be considered a disease biomarker. Materials and Methods; In 20 evaluable pts, aged 38 to 81 years (median 57.5), SCE and MN were measured both before and four weeks after conservative surgery. While, in those 10 pts who had previously received chemotherapy (CT), testing was done immediately before radiotherapy (RT). Further Controls were done 8 weeks after RT on all pts and at 6 months in 9 cases. All pts will be studied regularly during follow-up. There were 15 ductal infiltrant carcinomas (13 G2, 2 G3), 1 medullary carcinoma, 1 adenocarcinoma and 3 intraductal carcinomas with microinfiltration. Median tumor size was 12 mm (range 7-27 mm). In 16 and 11 cases estrogen and progestinic receptors were positive, respectively. Five pts had axillary positive nodes and in three of these cases they were four or more. Ten pts underwent adjuvant chemotherapy (CMF or FEC). Honnonotherapy was prescribed to 14 cases. RT was delivered to thè breast ± sovraclavicular nodes; single dose was 1.8-2 Gy, total dose 50.4-50 Gy; a 10 Gy boost was delivered to thè tumor bed. Student’s t test compared SCE and MN basai values to both those from a healthy control group of 7 women and those values obtained from treatment/follow-up times. Results:SCE value reduction after surgery, though not statistically significant, (p 0.07) seems to be a result of tumor removai while thè SCE increase after chemotherapy (p 0.04) is most likely to be a result of cytotoxic damage. MN increase after RT (p < 0.01) is most likely due to genotoxic damage. A statistically significant difference (p 0.04) was observed between SCE basai and control group values. Conclusions: The frequency of SCE as a cancer biomarker was confirmed by thè difference obtained comparing basai testing to control group values. Results here suggest that SCE and MN must be an index of damage due to CT and RT, respectively. In thè future, this study will seek to determine if SCE and MN frequency measurements during follow-up are disease progression predictors.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
