Chlorpyrifos is an organophosphorous insecticide whose main effect consists in the inhibition of acetylcholinesterase enzyme. It is very toxic for fish, but little is still known about its consequences on organs excluding the central nervous system. The goal of the present study was to evaluate the effects of CPF in gill, kidney and liver of feminine sub-adult specimens of rainbow trout. Behavioural observations were also carried out to confirm the absorption of the insecticide by the fish. The experiment lasted 96 h and was carried out in semi-static conditions, involving 170 individuals. 70 specimens were exposed to LC50 of CPF dissolved in ethanol, 50 to ethanol only and 50 weren't treated. Every 24 h, behavioural responses were detected, and then 15 fish were caught and subjected to anatomopathological exam. Samples of gills, liver and kidney were collected for histological analyses. Specimens exposed to CPF showed longer time of response and a gradual loss of movement control. The anatomopathological examination didn't highlight any gross lesion. The histological analyses revealed that the ethanol interfered with the detection of CPF effects on gills and kidney, while a mild turbid degeneration of the hepatocytes in liver was attributed to the sole insecticide.
Valutazione della sintomatologia, dei quadri anatomopatologici e istologici in trote iridea (Oncorhynchus mykiss) esposte a Chlorpyrifos in microcosmo
ELIA, Antonia Concetta;
2013
Abstract
Chlorpyrifos is an organophosphorous insecticide whose main effect consists in the inhibition of acetylcholinesterase enzyme. It is very toxic for fish, but little is still known about its consequences on organs excluding the central nervous system. The goal of the present study was to evaluate the effects of CPF in gill, kidney and liver of feminine sub-adult specimens of rainbow trout. Behavioural observations were also carried out to confirm the absorption of the insecticide by the fish. The experiment lasted 96 h and was carried out in semi-static conditions, involving 170 individuals. 70 specimens were exposed to LC50 of CPF dissolved in ethanol, 50 to ethanol only and 50 weren't treated. Every 24 h, behavioural responses were detected, and then 15 fish were caught and subjected to anatomopathological exam. Samples of gills, liver and kidney were collected for histological analyses. Specimens exposed to CPF showed longer time of response and a gradual loss of movement control. The anatomopathological examination didn't highlight any gross lesion. The histological analyses revealed that the ethanol interfered with the detection of CPF effects on gills and kidney, while a mild turbid degeneration of the hepatocytes in liver was attributed to the sole insecticide.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
