The use of aerial parts of Euphrasia pectinata Ten. usually known as Eyebright, is one of the commonest folk remedy in many European and Asian countries to treat eye problems. By a bioassay-guided fractionation of the dichloromethane fraction of E. pectinata methanolic extract, following the antiproliferative activity on different cancer cell lines (MCF-7, HeLa, LNCaP, ACHN and C32), the new iridoid euphrasin (1), and the known campsinol (2), artselaenin A (3), and artselaenin B (4) were isolated. The structure of euphrasin (1) was determined by spectroscopic methods. All compounds screened by SRB assay exhibited antiproliferative activity in a concentration dependent-manner. In particular, euphrasin (1) showed a promising activity against LNCaP and HeLa cells with IC50 values of 0.1 and 0.2 mM, respectively. Signficative results were also obtained with campsinol (2) with IC50 value of 1.9 mM on endometrial cancer cells.

Bioguided fractionation of Euphrasia pectinata Ten. and isolation of iridoids with antiproliferative activity

MARCOTULLIO, Maria Carla
Supervision
;
TEMPERINI, Andrea
Data Curation
;
CURINI, Massimo
Membro del Collaboration Group
2015

Abstract

The use of aerial parts of Euphrasia pectinata Ten. usually known as Eyebright, is one of the commonest folk remedy in many European and Asian countries to treat eye problems. By a bioassay-guided fractionation of the dichloromethane fraction of E. pectinata methanolic extract, following the antiproliferative activity on different cancer cell lines (MCF-7, HeLa, LNCaP, ACHN and C32), the new iridoid euphrasin (1), and the known campsinol (2), artselaenin A (3), and artselaenin B (4) were isolated. The structure of euphrasin (1) was determined by spectroscopic methods. All compounds screened by SRB assay exhibited antiproliferative activity in a concentration dependent-manner. In particular, euphrasin (1) showed a promising activity against LNCaP and HeLa cells with IC50 values of 0.1 and 0.2 mM, respectively. Signficative results were also obtained with campsinol (2) with IC50 value of 1.9 mM on endometrial cancer cells.
2015
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/1343467
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