The use of aerial parts of Euphrasia pectinata Ten. usually known as Eyebright, is one of the commonest folk remedy in many European and Asian countries to treat eye problems. By a bioassay-guided fractionation of the dichloromethane fraction of E. pectinata methanolic extract, following the antiproliferative activity on different cancer cell lines (MCF-7, HeLa, LNCaP, ACHN and C32), the new iridoid euphrasin (1), and the known campsinol (2), artselaenin A (3), and artselaenin B (4) were isolated. The structure of euphrasin (1) was determined by spectroscopic methods. All compounds screened by SRB assay exhibited antiproliferative activity in a concentration dependent-manner. In particular, euphrasin (1) showed a promising activity against LNCaP and HeLa cells with IC50 values of 0.1 and 0.2 mM, respectively. Signficative results were also obtained with campsinol (2) with IC50 value of 1.9 mM on endometrial cancer cells.
Bioassay-guided fractionation of Euphrasia pectinata Ten. and isolation of iridoids with antiproliferative activity
MARCOTULLIO, Maria Carla
Supervision
;TEMPERINI, AndreaMembro del Collaboration Group
;CURINI, MassimoMembro del Collaboration Group
2015
Abstract
The use of aerial parts of Euphrasia pectinata Ten. usually known as Eyebright, is one of the commonest folk remedy in many European and Asian countries to treat eye problems. By a bioassay-guided fractionation of the dichloromethane fraction of E. pectinata methanolic extract, following the antiproliferative activity on different cancer cell lines (MCF-7, HeLa, LNCaP, ACHN and C32), the new iridoid euphrasin (1), and the known campsinol (2), artselaenin A (3), and artselaenin B (4) were isolated. The structure of euphrasin (1) was determined by spectroscopic methods. All compounds screened by SRB assay exhibited antiproliferative activity in a concentration dependent-manner. In particular, euphrasin (1) showed a promising activity against LNCaP and HeLa cells with IC50 values of 0.1 and 0.2 mM, respectively. Signficative results were also obtained with campsinol (2) with IC50 value of 1.9 mM on endometrial cancer cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.