A robust liquid chromatography–tandem mass spectrometry (LC–MS-MS) method has been developedandvalidatedforthedeterminationoflidocaine(LIDO)anditsmetabolite(monoethylglycinexylidide (MEGX)) in serum. One hundred microliters of bovine serum were spiked with LIDO-d10 as internal standard and deproteinized with acetonitrile prior to solid-phase extraction purification using strong cation exchange cartridge. The chromatographic separation was achieved on a BetaBasic-18 column with a mobile phase consisting of aqueous 0.1% formic acid and 0.1% formic acid in acetonitrile. The instrumental linearity was verified from 0.4 to 1,000 ng/mL obtaining determination coefficients (r2) of >0.99. The limit of quantification (LOQ) was set at 1 ng/mL for both LIDO and MEGX. The coefficients of variation for within- and between-batch imprecision, including LOQ, were≤10%andthepercentageofinaccuracywas<15%.Theabsoluterecoverieswere>75%forboth analytes.Experimentsdemonstratedthemethodapplicabilitytoseraofdifferentanimalspeciesand also to plasma, urine and milk matrices.
Flexible Method for Analysis of Lidocaine and its Metabolite in Biological Fluids
DI SALVO, Alessandra;
2016
Abstract
A robust liquid chromatography–tandem mass spectrometry (LC–MS-MS) method has been developedandvalidatedforthedeterminationoflidocaine(LIDO)anditsmetabolite(monoethylglycinexylidide (MEGX)) in serum. One hundred microliters of bovine serum were spiked with LIDO-d10 as internal standard and deproteinized with acetonitrile prior to solid-phase extraction purification using strong cation exchange cartridge. The chromatographic separation was achieved on a BetaBasic-18 column with a mobile phase consisting of aqueous 0.1% formic acid and 0.1% formic acid in acetonitrile. The instrumental linearity was verified from 0.4 to 1,000 ng/mL obtaining determination coefficients (r2) of >0.99. The limit of quantification (LOQ) was set at 1 ng/mL for both LIDO and MEGX. The coefficients of variation for within- and between-batch imprecision, including LOQ, were≤10%andthepercentageofinaccuracywas<15%.Theabsoluterecoverieswere>75%forboth analytes.Experimentsdemonstratedthemethodapplicabilitytoseraofdifferentanimalspeciesand also to plasma, urine and milk matrices.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
