This paper presents the fabrication and testing of a lab-on-chip system suitable for treatment of DNA. It includes two main modules: a system-on-glass (SoG) and a disposable microfuidic chip. The SoG integrates, on the same glass substrate, thin fIlm metal heaters and amorphous silicon temperature sensors to achieve a uniform temperature distribution (within ±l°C) in the heated area. Two polydimethylsiloxane microtluidic chips have been developed: a PCR-Chip for DNA amplification and a dsDNA-Chip for separation and selective isolation of a ssDNA from a dsDNA. The proposed system aims therefore to develop compact, lowcost devices that can implement multiple functions in biochemical procedures. In particular, the tested bioanalytical procedures are well suited for carrying-out an on-chip SELEX process, a combinatorial chemistry technique for the selection of aptamers.
Lab-on-glass system for DNA treatments
Placidi, P.;Scorzoni, A.
2017
Abstract
This paper presents the fabrication and testing of a lab-on-chip system suitable for treatment of DNA. It includes two main modules: a system-on-glass (SoG) and a disposable microfuidic chip. The SoG integrates, on the same glass substrate, thin fIlm metal heaters and amorphous silicon temperature sensors to achieve a uniform temperature distribution (within ±l°C) in the heated area. Two polydimethylsiloxane microtluidic chips have been developed: a PCR-Chip for DNA amplification and a dsDNA-Chip for separation and selective isolation of a ssDNA from a dsDNA. The proposed system aims therefore to develop compact, lowcost devices that can implement multiple functions in biochemical procedures. In particular, the tested bioanalytical procedures are well suited for carrying-out an on-chip SELEX process, a combinatorial chemistry technique for the selection of aptamers.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
