Abstract: The homeobox genes are present in the genome of the honeybee Apis mellifera and their expression has been studied principally during embryonic development. The aim of this research was to evaluate expression of three key hox genes from the larval period to the adult. The expression of honeybee GB51301, Ant and Ubx were examined in 24 and 72 hrs larvae, and pupa, classified on the basis of eye colours, using quantitative RT PCR on either the entire body or the head only. The results show that levels of expression from these genes change in relation to the development of various organs. GB51301(HOX3/zen) expression is mainly localised in the head and is expressed in the larvae when the first nervous cells are formed and in the adult worker. ANTP is expressed in the white-eye pupa at a similar time to the development of antenna. UBX is also expressed in white and red eye pupae at a similar time to leg formation. The expression of these genes is practically absent in the brown eye pupa when the development of these organs is completed. These genes are found mainly in the body.
Expression of Hox Genes, Zen (GB 51301) ANT and UBX during the Development of Honey Bee (Apis mellifera)
Samuela Cataldi
2014
Abstract
Abstract: The homeobox genes are present in the genome of the honeybee Apis mellifera and their expression has been studied principally during embryonic development. The aim of this research was to evaluate expression of three key hox genes from the larval period to the adult. The expression of honeybee GB51301, Ant and Ubx were examined in 24 and 72 hrs larvae, and pupa, classified on the basis of eye colours, using quantitative RT PCR on either the entire body or the head only. The results show that levels of expression from these genes change in relation to the development of various organs. GB51301(HOX3/zen) expression is mainly localised in the head and is expressed in the larvae when the first nervous cells are formed and in the adult worker. ANTP is expressed in the white-eye pupa at a similar time to the development of antenna. UBX is also expressed in white and red eye pupae at a similar time to leg formation. The expression of these genes is practically absent in the brown eye pupa when the development of these organs is completed. These genes are found mainly in the body.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
