Platelet-derived growth factor A (PDGF-A) is implicated in hair follicle (HF) morphogenesis and hair cycle. It is considered an extrinsic signal, secreted by intradermal adipose tissue that can activate the hair follicle stem cells. Furthermore, PDGF-A is produced by the epidermis and follic-ular epithelium in humans. In this study, PDGF-A expression was investigated in the skin of the cashmere goats with the aim to correlate this molecule with HF stem compartment. Skin samples were collected from seven goats during different times according to hair cycle: in particular, sam-pling were performed at early anagen, anagen, early catagen and last catagen. For the immuno-histochemistry, a mouse monoclonal anti PDGF-A antibody (SCBT) was used. Immunostaining was observed in some structures of the goat skin including the epidermis, HFs, blood vessels, arrector pili muscles and myoepithelial cells of the sebaceous gland. In particular, positive cells were localized in the basal layer of the epidermis and of the HF outer root sheath. As regard HF, the staining extended from the infundibulum to the isthmus while no reaction was detected in the bulb. A stronger immunoreaction was observed in the bulge region of regressive HFs compared to anagen phase. The expression of PDGF-A by the follicular epithelium and the increased im-munoreaction during the regressive phase of the hair cycle let us to suppose that the PDGF-A, secreted by the HF, is involved in the anagen activation during the hair cycle.
Platelet-derived growth factor-A is expressed by hair follicle epithelium in cashmere goats
Mercati, Francesca;Dall’Aglio, Cecilia;Pascucci, Luisa;Ceccarelli, Piero.
2017
Abstract
Platelet-derived growth factor A (PDGF-A) is implicated in hair follicle (HF) morphogenesis and hair cycle. It is considered an extrinsic signal, secreted by intradermal adipose tissue that can activate the hair follicle stem cells. Furthermore, PDGF-A is produced by the epidermis and follic-ular epithelium in humans. In this study, PDGF-A expression was investigated in the skin of the cashmere goats with the aim to correlate this molecule with HF stem compartment. Skin samples were collected from seven goats during different times according to hair cycle: in particular, sam-pling were performed at early anagen, anagen, early catagen and last catagen. For the immuno-histochemistry, a mouse monoclonal anti PDGF-A antibody (SCBT) was used. Immunostaining was observed in some structures of the goat skin including the epidermis, HFs, blood vessels, arrector pili muscles and myoepithelial cells of the sebaceous gland. In particular, positive cells were localized in the basal layer of the epidermis and of the HF outer root sheath. As regard HF, the staining extended from the infundibulum to the isthmus while no reaction was detected in the bulb. A stronger immunoreaction was observed in the bulge region of regressive HFs compared to anagen phase. The expression of PDGF-A by the follicular epithelium and the increased im-munoreaction during the regressive phase of the hair cycle let us to suppose that the PDGF-A, secreted by the HF, is involved in the anagen activation during the hair cycle.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
