Amplified fragment length polymorphism (AFLP) was used to study diversity among Lebanese olive trees varieties in Bshaaleh. 15 olive trees coming from Bshaaleh were genotyped using 5 AFLP primer combinations. A total of 332 amplification products and 153 polymorphic fragments were scored, with a percentage of polymorphism ranging from 33% (E-CCA / M-ACC) to 51% (E-CAC / M-ACA) depending on primer combination. Low correlation (r<0.7) between the primer combination E-CCA/M-ACC and all other pairs was noted. Results were analyzed for similarity among Bshaaleh accessions via unweighted pair group means cluster analysis, resulting in 2 clusters corresponding to named variety designations. Similarity coefficients of 0.965 to 1.000 among accessions were observed indicating the possible presence of varieties. Intravarietal similarity was more than 0.99 for 93% of the accessions. Only the accession tree B3 showed a relatively low genetic similarity to the cluster defined and could be considered as outlier. The cophenetic correlation between the dendrogram and the similarity matrix revealed a very good degree of fit (r=0.994). According to the Principal Coordinate Analysis, discrimination analysis revealed similar findings and grouped trees into one main group and other unrelated accessions and discriminated at 83% by the first three axes, suggesting that all the ancient olive trees of Bshaaleh are related to the same variety. The findings of this study pointed for the need to plan for a sampling strategy that takes into consideration geographic provenances.
Molecular characterization of ancient olive trees in Bshaaleh Lebanon.
Fabio VeronesiMembro del Collaboration Group
2018
Abstract
Amplified fragment length polymorphism (AFLP) was used to study diversity among Lebanese olive trees varieties in Bshaaleh. 15 olive trees coming from Bshaaleh were genotyped using 5 AFLP primer combinations. A total of 332 amplification products and 153 polymorphic fragments were scored, with a percentage of polymorphism ranging from 33% (E-CCA / M-ACC) to 51% (E-CAC / M-ACA) depending on primer combination. Low correlation (r<0.7) between the primer combination E-CCA/M-ACC and all other pairs was noted. Results were analyzed for similarity among Bshaaleh accessions via unweighted pair group means cluster analysis, resulting in 2 clusters corresponding to named variety designations. Similarity coefficients of 0.965 to 1.000 among accessions were observed indicating the possible presence of varieties. Intravarietal similarity was more than 0.99 for 93% of the accessions. Only the accession tree B3 showed a relatively low genetic similarity to the cluster defined and could be considered as outlier. The cophenetic correlation between the dendrogram and the similarity matrix revealed a very good degree of fit (r=0.994). According to the Principal Coordinate Analysis, discrimination analysis revealed similar findings and grouped trees into one main group and other unrelated accessions and discriminated at 83% by the first three axes, suggesting that all the ancient olive trees of Bshaaleh are related to the same variety. The findings of this study pointed for the need to plan for a sampling strategy that takes into consideration geographic provenances.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
