Background and Objective: In Vietnam, “Dai trang hoan ba Giang” (BG), which belongs to herbalist “Ba Giang”, has been one of the famous herbal remedies for ulcerative colitis and diarrhea for over 100 years. However, up to the present, the main BG’s chemical constituents have not been investigated. Therefore, this study aims to investigate the phytochemistry and antiproliferative activity of isolated compounds from BG on Acute Myeloid Leukemia (OCI-AML) cells as well as evaluating its safety by the acute and subchronic toxicological tests. Methods: Compounds from herbmaterial were isolated by using column chromatography. Their structures were determined by combining spectral analysis and comparison with reported data. The concentrations of components were were determined by HPLC/DAD analysis. Anti-proliferative activity in OCI-AML cell line of isolated compounds was carried out by flow cytometry analysis. The acute and subchronic oral toxicity in micewere appraised by observingdaily for clinical manifestations of toxicity while the experiment lasted. Results: In this study, driedpowders of materials were extracted by 70% EtOH to afford an extract (DTBG). Two compounds, including palmatine (1) and berberine (2) were isolated from DTBG. The DTBG inhibited the growth of OCI-AML by a significant increase of caspase-8-independent apoptosis as measured by propidium iodide flow cytometry analysis and caspase-3 activation. In the acute study, DTBG is well tolerated, non-toxic and non-lethal to mice under the present experimental conditions. In thesubchronic study in mice, DTBG did not impact on weight gain, blood, liver and kidney function. Conclusion: Overall, the main chemical constituents of DTBG and its effect on proliferation of acute myeloid leukemia cells were reported for the first time. Moreover, the results suggest that, DTBG did not express any significant toxic effect in mice. Hence, the extract can be utilized for pharmaceutical formulations.

The alkaloids and bioactivities of ethanol extract from a traditional remedy of Vietnam

Adorisio S.;Delfino D. V.
2020

Abstract

Background and Objective: In Vietnam, “Dai trang hoan ba Giang” (BG), which belongs to herbalist “Ba Giang”, has been one of the famous herbal remedies for ulcerative colitis and diarrhea for over 100 years. However, up to the present, the main BG’s chemical constituents have not been investigated. Therefore, this study aims to investigate the phytochemistry and antiproliferative activity of isolated compounds from BG on Acute Myeloid Leukemia (OCI-AML) cells as well as evaluating its safety by the acute and subchronic toxicological tests. Methods: Compounds from herbmaterial were isolated by using column chromatography. Their structures were determined by combining spectral analysis and comparison with reported data. The concentrations of components were were determined by HPLC/DAD analysis. Anti-proliferative activity in OCI-AML cell line of isolated compounds was carried out by flow cytometry analysis. The acute and subchronic oral toxicity in micewere appraised by observingdaily for clinical manifestations of toxicity while the experiment lasted. Results: In this study, driedpowders of materials were extracted by 70% EtOH to afford an extract (DTBG). Two compounds, including palmatine (1) and berberine (2) were isolated from DTBG. The DTBG inhibited the growth of OCI-AML by a significant increase of caspase-8-independent apoptosis as measured by propidium iodide flow cytometry analysis and caspase-3 activation. In the acute study, DTBG is well tolerated, non-toxic and non-lethal to mice under the present experimental conditions. In thesubchronic study in mice, DTBG did not impact on weight gain, blood, liver and kidney function. Conclusion: Overall, the main chemical constituents of DTBG and its effect on proliferation of acute myeloid leukemia cells were reported for the first time. Moreover, the results suggest that, DTBG did not express any significant toxic effect in mice. Hence, the extract can be utilized for pharmaceutical formulations.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/1464772
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