DEVELOPMENT OF IN VITRO MULTIPLICATION METHOD FOR Bidens pilosa L.

Bidens pilosa L. it is a medicinal plant which is often used traditionally in the treatment of several diseases. Many pharmaceutical products from this plant have been investigated and these are produced from a very large quantity of plant parts. However, the high production of biomass for this plant is requested. In vitro micropropagation may be a valuable solution to obtain multiple uniform plants in a short period of time. This study has been conducted to develop such specific in vitro multiplication protocol for Bidens pilosa L. The impacts of basal medium combined with different plants growth hormone at various dose have been examined for thedevelopment of three explants (shoot with one nod, leaves and cotyledons). The cotyledons and leaves transplanted in all tested culture medium didn't show any development during this experiment. The utmost shoot elongation and the many number of roots from shoot tip explants were achieved on the culture media containing IA and NNA. IA at 1 mg/l increased the shoots length at 11.1 cmwith 17 rootsfollowed by MA at 0.5 mg/l (8.3 cm with 12 roots) and NAA at 0.5 mg/l (7.1 cm with 10 roots). The top number of the leaves was obtained with the association of BAP and IAA (23 leaves), BAP and NAA (20 leaves) at 0.5 mg/l concentrationand the shoots had 7 roots. BAP applied alone at 0.5 mg/l showed a meaningfully role in leaves multiplication (shoots have 17 leaves) but less effective in lengthening the shoots and roots initiation (shoots have 4 cm with 3 roots). The BAP used alone and in combination at 1.0 mg/l inhibit the plant growth. Healthy B. pilosa plants with roots were obtained after 23 days with the help of in-vitro culture and the plants sent in the greenhouse have been successfully adapted to the field conditions.