Reversed-phase high-performance liquid chromatography (RP-HPLC) is more and more adopted as a fruitful too[ for the estimation of several physico-chemical properties of diverse classes of organic compounds. In this frame, derived chromatographic indices have been proposed a's effective parameters to measure the lipophilicity, (log P or log D) of compounds. Instead, a limited attention has been directed towards the chromatographic evaluation of the Critical Micellar Concentration (CMC), one of the most important parameters employed to study the bile acid physico-chemical profile. We have recently reported on the effectiveness of the derived chromatographic index phi(0) for the study of the self-aggregation process of bile acids. Here we show that this index is independent upon the adopted chromatographic environment so as to be instrumental for the evaluation of the hydrophobic/hydrophilic balance of bile acids. Molecular modelling studies have also been undertaken with the aim of rationalizing the experimental findings.

Derived chromatographic indices as effective tools to study the self-aggregation process of bile acids

NATALINI, Benedetto;SARDELLA, Roccaldo;CAMAIONI, Emidio;MACCHIARULO, Antonio;GIOIELLO, ANTIMO;PELLICCIARI, Roberto
2009

Abstract

Reversed-phase high-performance liquid chromatography (RP-HPLC) is more and more adopted as a fruitful too[ for the estimation of several physico-chemical properties of diverse classes of organic compounds. In this frame, derived chromatographic indices have been proposed a's effective parameters to measure the lipophilicity, (log P or log D) of compounds. Instead, a limited attention has been directed towards the chromatographic evaluation of the Critical Micellar Concentration (CMC), one of the most important parameters employed to study the bile acid physico-chemical profile. We have recently reported on the effectiveness of the derived chromatographic index phi(0) for the study of the self-aggregation process of bile acids. Here we show that this index is independent upon the adopted chromatographic environment so as to be instrumental for the evaluation of the hydrophobic/hydrophilic balance of bile acids. Molecular modelling studies have also been undertaken with the aim of rationalizing the experimental findings.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/149820
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