ChrX markers represent an efficient supplementation of autosomal and Y-chromosomal STR analysis and ChrX haplotyping can elucidate complicated kinship situations. This is the reason why it needs to increase the population data for ChrX STR allelic frequencies and to create national or local databases. An esaplex PCR was developed to amplify DXS6789, HumARA, DXS7423, DXS6807, DXS101 and DXS8377 in some Italian samples from Perugia and Terni. This system represents a protocol for the ChrX analysis with a shorter procedure. The DNA was extracted from 100 blood samples using the QIAmp DNA Minikit produced by Qiagen. The samples were detected on an ABI PRISM 310 Genetic Analyzer (Applied Biosystems), using the same dye labels, run conditions, standard (GeneScan 500 Liz) and matrix file of AmpFlSTRIdentifiler. We performed statistical analysis for all the loci.
Allele distribution of 6 X-chromosome STR loci in an Italian population sample
LANCIA, MASSIMO;BACCI, Mauro
2006
Abstract
ChrX markers represent an efficient supplementation of autosomal and Y-chromosomal STR analysis and ChrX haplotyping can elucidate complicated kinship situations. This is the reason why it needs to increase the population data for ChrX STR allelic frequencies and to create national or local databases. An esaplex PCR was developed to amplify DXS6789, HumARA, DXS7423, DXS6807, DXS101 and DXS8377 in some Italian samples from Perugia and Terni. This system represents a protocol for the ChrX analysis with a shorter procedure. The DNA was extracted from 100 blood samples using the QIAmp DNA Minikit produced by Qiagen. The samples were detected on an ABI PRISM 310 Genetic Analyzer (Applied Biosystems), using the same dye labels, run conditions, standard (GeneScan 500 Liz) and matrix file of AmpFlSTRIdentifiler. We performed statistical analysis for all the loci.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
