DNA-stabilized silver nanoclusters (DNA-AgNCs) have promising properties for biosensing and bioimaging applications. However, in order to enhance the applicability in these fields, a detailed understanding of the interactions that occur between DNA-AgNCs and other biomolecules is essential. In this work, the spectroscopic properties of red-emitting DNA-AgNCs have been investigated in the presence of three biomolecules, namely lysozyme, bovine serum albumin and ss-DNA, which have been selected in order to have either a different biological nature (two proteins and one nucleic acid) or different net charge at physiological pH (one positive and two negative). The systematic characterization of the steady-state absorption and emission spectra, combined with time-resolved fluorescence and anisotropy decay measurements, of DNA-AgNCs in the presence of each biomolecule, allowed to establish the nature of the interactions that affect the photophysical properties of the DNA-AgNCs.

Probing the Fluorescence Behavior of DNA-Stabilized Silver Nanoclusters in the Presence of Biomolecules

Gambucci M.;Zampini G.;Quaglia G.;Latterini L.
2021

Abstract

DNA-stabilized silver nanoclusters (DNA-AgNCs) have promising properties for biosensing and bioimaging applications. However, in order to enhance the applicability in these fields, a detailed understanding of the interactions that occur between DNA-AgNCs and other biomolecules is essential. In this work, the spectroscopic properties of red-emitting DNA-AgNCs have been investigated in the presence of three biomolecules, namely lysozyme, bovine serum albumin and ss-DNA, which have been selected in order to have either a different biological nature (two proteins and one nucleic acid) or different net charge at physiological pH (one positive and two negative). The systematic characterization of the steady-state absorption and emission spectra, combined with time-resolved fluorescence and anisotropy decay measurements, of DNA-AgNCs in the presence of each biomolecule, allowed to establish the nature of the interactions that affect the photophysical properties of the DNA-AgNCs.
2021
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11391/1500733
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