Abstract: Background: The restricted availability of cadaveric human donor pancreases mandates validation of possibly inexhaustible, alternative sources of insulin secretory cells in order to expand islet transplant for the therapy of insulin dependent diabetes mellitus (T1DM). Methods: Neonatal pig pancreatic islets (NPI), isolated and purified by our method, were specially cultured until confluent cell monolayers were obtained. Expression of several b-cell phenotype transcriptional factors, under glucose and other stimuli, were examined throughout 90 days of culture. Results: High glucose concentration and glucagon-like peptide 1 (GLP- 1) were associated with maintenance either of insulin secretory patterns from the incubated cell monolayers, or expression of transcriptional markers associated with b-cell like phenotypes. Conclusion: Morphological and molecular expression of b-cell markers and products from NPI cell monolayers seem to identify a novel and potentially powerful source of insulin producing cells that might fulfill transplant needs for insulin substitution therapy
Long-term cultured neonatal porcine islet cell monolayers: a potential tissue source for transplant in diabetes.
MANCUSO, FRANCESCA;CALVITTI, Mario;LUCA, Giovanni;MONTANUCCI, Pia;BECCHETTI, Ennio;CALAFIORE, Riccardo
2006
Abstract
Abstract: Background: The restricted availability of cadaveric human donor pancreases mandates validation of possibly inexhaustible, alternative sources of insulin secretory cells in order to expand islet transplant for the therapy of insulin dependent diabetes mellitus (T1DM). Methods: Neonatal pig pancreatic islets (NPI), isolated and purified by our method, were specially cultured until confluent cell monolayers were obtained. Expression of several b-cell phenotype transcriptional factors, under glucose and other stimuli, were examined throughout 90 days of culture. Results: High glucose concentration and glucagon-like peptide 1 (GLP- 1) were associated with maintenance either of insulin secretory patterns from the incubated cell monolayers, or expression of transcriptional markers associated with b-cell like phenotypes. Conclusion: Morphological and molecular expression of b-cell markers and products from NPI cell monolayers seem to identify a novel and potentially powerful source of insulin producing cells that might fulfill transplant needs for insulin substitution therapyI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
