Oxidative stress may cause severe cellular damage to both allo- and xeno-transplanted islets, additional to islet graft-directed immunity, in diabetic patients. We thus aimed to examine the effects of antioxidants on in vitro culture-maintained, neonatal porcine cell clusters (NPCCs). NPCCs were treated with antioxidants (vitamins D3 and E) by a certain time of their maturation and differentiation process. Insulin recovery showed that both vitamins D3 and E, unlike untreated controls, resulted in preserv ation of the islet function for significantly long periods of time.
Effects of Anti-Oxidizing Vitamins on in Vitro Cultured Porcine Neonatal Pancreatic Islet Cells
LUCA, Giovanni;NASTRUZZI, Claudio;BROZZETTI, Annalisa;SATURNI, ALESSANDRO;MUGHETTI, Debora;RICCI, Maurizio;ROSSI, Carlo;BRUNETTI, Paolo;CALAFIORE, Riccardo
2000
Abstract
Oxidative stress may cause severe cellular damage to both allo- and xeno-transplanted islets, additional to islet graft-directed immunity, in diabetic patients. We thus aimed to examine the effects of antioxidants on in vitro culture-maintained, neonatal porcine cell clusters (NPCCs). NPCCs were treated with antioxidants (vitamins D3 and E) by a certain time of their maturation and differentiation process. Insulin recovery showed that both vitamins D3 and E, unlike untreated controls, resulted in preserv ation of the islet function for significantly long periods of time.File in questo prodotto:
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