During 2006, surveys on approximately 92 ha of Umbrian (central Italy) sunflower fields were carried out for monitoring distribution and race variability of the pathogen. Twelve fields planted with commercial hybrids were surveyed. Downy mildew was only observed in five fields, with 2 to 3% of disease incidence. Pathogen inocula were directly recovered from infected leaves by brushing the fungal structures or after infected leaves were incubated in a humid chamber at 18 to 20°C in the dark for 24 to 48 h. After increasing initial inocula on the suscpetible cv. Ala, race identification of four isolates was determined by the reaction of three standard sets of nine differential sunflower lines using a triplet code. The isolates were also evaluated for their sensitivity to metalaxyl-M (Apron XL 31.8%) used at the Italian registered rate (1.05 g of a.i. per kg of seed). Three isolates were characterized as race 700 and one as a mixture of races 700 and 704. To our knowledge, this is the first report of race 704 in Italy. All field isolates were also metalaxyl-M sensitive as already reported in other European countries.
A new race, 704, of Plasmopara helianthi pathogen of sunflower downy mildew in Italy
TOSI, Laura;BECCARI, GIOVANNI
2007
Abstract
During 2006, surveys on approximately 92 ha of Umbrian (central Italy) sunflower fields were carried out for monitoring distribution and race variability of the pathogen. Twelve fields planted with commercial hybrids were surveyed. Downy mildew was only observed in five fields, with 2 to 3% of disease incidence. Pathogen inocula were directly recovered from infected leaves by brushing the fungal structures or after infected leaves were incubated in a humid chamber at 18 to 20°C in the dark for 24 to 48 h. After increasing initial inocula on the suscpetible cv. Ala, race identification of four isolates was determined by the reaction of three standard sets of nine differential sunflower lines using a triplet code. The isolates were also evaluated for their sensitivity to metalaxyl-M (Apron XL 31.8%) used at the Italian registered rate (1.05 g of a.i. per kg of seed). Three isolates were characterized as race 700 and one as a mixture of races 700 and 704. To our knowledge, this is the first report of race 704 in Italy. All field isolates were also metalaxyl-M sensitive as already reported in other European countries.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.