J Med Vet Mycol. 1997 Jul-Aug;35(4):299-303. Identification of a 105 kilodalton Cryptococcus neoformans mannoprotein involved in human cell-mediated immune response. Pitzurra L, Vecchiarelli A, Peducci R, Cardinali A, Bistoni F. SourceDepartment of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy. Abstract In the present study, using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blot techniques, the patterns of proteins extracted from C. neoformans yeast cells were analysed. A major 105 kilodalton (kDa) antigen that binds to Concanavalin A and cross-reacts with anti-mannan antibodies was identified. The 105 kDa mannoprotein, highly expressed in the acapsular mutant of C. neoformans with respect to the encapsulated strain, is involved in the lymphoproliferative response of T lymphocytes to Cryptococcus-sensitized monocytes. PMID: 9292430
Identification of a 105 kilodalton Cryptococcus neoformans mannoprotein involved in human cell-mediated immune response.
PITZURRA, Lucia;VECCHIARELLI, Anna;BISTONI, Francesco
1997
Abstract
J Med Vet Mycol. 1997 Jul-Aug;35(4):299-303. Identification of a 105 kilodalton Cryptococcus neoformans mannoprotein involved in human cell-mediated immune response. Pitzurra L, Vecchiarelli A, Peducci R, Cardinali A, Bistoni F. SourceDepartment of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy. Abstract In the present study, using sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blot techniques, the patterns of proteins extracted from C. neoformans yeast cells were analysed. A major 105 kilodalton (kDa) antigen that binds to Concanavalin A and cross-reacts with anti-mannan antibodies was identified. The 105 kDa mannoprotein, highly expressed in the acapsular mutant of C. neoformans with respect to the encapsulated strain, is involved in the lymphoproliferative response of T lymphocytes to Cryptococcus-sensitized monocytes. PMID: 9292430I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
