The hydrogel thiopropyl-sepharose 6B was used as a model matrix system to bind the natural cytotoxic sesquiterpene lactones grosheimin 1 and deacylcynaropicrin 2. The hydrogel thio groups were bonded to the lactone exomethylene groups and the release of the lactones from the polymer was studied. Since it is thought that cancer cells have a higher oxidative potential than normal cells, the release was carried out through an oxidative cleavage. Different concentrations of hydrogen peroxide solutions were added to the lactone-polymer conjugates, thus, imitating in vivo conditions. The results obtained showed that both the rate and amount of grosheimin and deacylcynaropicrin release depend linearly on the H2O2 concentration.
Thiol gel as a matrix for binding-release of a,b-unsaturated sesquiterpene lactones
FARDELLA, Giuseppe;GRANDOLINI, Giuliano;AMBROGI, Valeria;CHIAPPINI, Ione
1999
Abstract
The hydrogel thiopropyl-sepharose 6B was used as a model matrix system to bind the natural cytotoxic sesquiterpene lactones grosheimin 1 and deacylcynaropicrin 2. The hydrogel thio groups were bonded to the lactone exomethylene groups and the release of the lactones from the polymer was studied. Since it is thought that cancer cells have a higher oxidative potential than normal cells, the release was carried out through an oxidative cleavage. Different concentrations of hydrogen peroxide solutions were added to the lactone-polymer conjugates, thus, imitating in vivo conditions. The results obtained showed that both the rate and amount of grosheimin and deacylcynaropicrin release depend linearly on the H2O2 concentration.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
