Cell Immunol. 1996 Apr 10;169(1):113-6. Tetanus toxin-sensitive VAMP-related proteins are present in murine macrophages. Pitzurra L, Rossetto O, Chimienti AR, Blasi E, Bistoni F. SourceDepartment of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy. Abstract The light chain of tetanus neurotoxin (TeTx) is a zinc endopeptidase specific for VAMP/synaptobrevin (VAMP), a 120-amino-acid integral protein previously described in the small synaptic vesicles of neuronal cells. TeTx has been shown to be active also on nonneuronal cells. By SDS-PAGE and quantitative immunoblotting on proteins derived from murine macrophages (Mphi) exposed to TeTx, we have shown that: (1) VAMP-related proteins are also present in Mphi and (2) such proteins are sensitive to TeTx proteolytic cleavage. The demonstration that TeTx acts on VAMP-related proteins also in Mphi offers a new and useful tool for molecular studies on Mphi exocytosis. PMID: 8612283
Tetanus toxin-sensitive VAMP-related proteins are present in murine macrophages.
PITZURRA, Lucia;BISTONI, Francesco
1996
Abstract
Cell Immunol. 1996 Apr 10;169(1):113-6. Tetanus toxin-sensitive VAMP-related proteins are present in murine macrophages. Pitzurra L, Rossetto O, Chimienti AR, Blasi E, Bistoni F. SourceDepartment of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy. Abstract The light chain of tetanus neurotoxin (TeTx) is a zinc endopeptidase specific for VAMP/synaptobrevin (VAMP), a 120-amino-acid integral protein previously described in the small synaptic vesicles of neuronal cells. TeTx has been shown to be active also on nonneuronal cells. By SDS-PAGE and quantitative immunoblotting on proteins derived from murine macrophages (Mphi) exposed to TeTx, we have shown that: (1) VAMP-related proteins are also present in Mphi and (2) such proteins are sensitive to TeTx proteolytic cleavage. The demonstration that TeTx acts on VAMP-related proteins also in Mphi offers a new and useful tool for molecular studies on Mphi exocytosis. PMID: 8612283I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.