Preliminary approach to mycorrhization of micropropagated linden sprouts with Tuber borchii.

l it reaches the suitable size for the next rooting phase on soil. The rooting method on soil is now a widespread system and is defined as "mixed vitro-vivo system". The main advantage is to obtain roots that are structurally much more similar to the definitive and partially functional root systems. The inductive treatment with IBA powdered has allowed to obtain 58% of the rooted shoots, while in the remaining 42% of the cases, the characteristic enlargement of the part of tissue treated with auxin has been recorded. The rooted shoots have developed an average of 2.1 roots/shoot with an average length of 16.6 mm. Finally, already after 4 months from the inoculum, it was observed up to 80% of mycorrhization with T. borchii and after 6 months an average of 45% of mycorrhization, by morphological analysis. These results, although still at the preliminary stage, are very interesting and promising in order to obtain micropropagated and mycorrhized lindens with T. borchii.