Genomic clones of the human GM2 activator protein have been isolated and analyzed. The 5 ' region of the gene demonstrated promoter activity as ascertained by its ability to drive luciferase gene expression in transfected COS cells. This sequence contains GC rich region and several putative promoter elements were present, including Spl, AP2, cAMP-responsive element, and B-cell-specific activating protein. Analysis of tissue distribution of the GM2 activator protein gene revealed tissue-specific variations in transcript levels. Placenta, bone marrow, mammary gland, bladder, lymph node, and spleen had the highest mRNA levels.
Promoter characterization and expression of the gene coding for the human gm2 activator protein
BECCARI, Tommaso;BALDUCCI, CHIARA;AISA, Maria Cristina;DELLA FAZIA, Maria Agnese;SERVILLO, Giuseppe;ORLACCHIO, Aldo
2001
Abstract
Genomic clones of the human GM2 activator protein have been isolated and analyzed. The 5 ' region of the gene demonstrated promoter activity as ascertained by its ability to drive luciferase gene expression in transfected COS cells. This sequence contains GC rich region and several putative promoter elements were present, including Spl, AP2, cAMP-responsive element, and B-cell-specific activating protein. Analysis of tissue distribution of the GM2 activator protein gene revealed tissue-specific variations in transcript levels. Placenta, bone marrow, mammary gland, bladder, lymph node, and spleen had the highest mRNA levels.File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
