Human interleukin 8 (IL-8) is an anti-inflammatory cyto- kine. A significant increase of the IL-8 concentration has been found in the plasma of marathon runners (Suzuki et al., 2000) and a similar upregulation of this interleukin was recently detected in lymphocytes of horses immediately after intense exercise (Cappelli, Verini-Supplizi, Capomaccio and Silves- trelli unpublished results). Equus caballus (ECA) IL8 full length cDNA, isolated from peripheral blood lymphocytes of an endurance horse, was sequenced (GenBank accession number AY184956). The mRNA sequence is 1089 nt in length and contains a 306 nt region that putatively codes for a 101 amino acid protein. In the framework of studying the modulation of gene expression profiles in racing horses, we report here the chromosomal location of ECA IL8. This result adds a new type I marker to the FISH map of the horse.
Assignement of the Equus caballus interleukin 8 gene(IL8)to chromosome 3q14.2 -q14.3 by in situ hybridization
CAPPELLI, Katia;VERINI SUPPLIZI, Andrea;
2006
Abstract
Human interleukin 8 (IL-8) is an anti-inflammatory cyto- kine. A significant increase of the IL-8 concentration has been found in the plasma of marathon runners (Suzuki et al., 2000) and a similar upregulation of this interleukin was recently detected in lymphocytes of horses immediately after intense exercise (Cappelli, Verini-Supplizi, Capomaccio and Silves- trelli unpublished results). Equus caballus (ECA) IL8 full length cDNA, isolated from peripheral blood lymphocytes of an endurance horse, was sequenced (GenBank accession number AY184956). The mRNA sequence is 1089 nt in length and contains a 306 nt region that putatively codes for a 101 amino acid protein. In the framework of studying the modulation of gene expression profiles in racing horses, we report here the chromosomal location of ECA IL8. This result adds a new type I marker to the FISH map of the horse.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
