Nine microsatellite markers for Cynara cardunculus L. were developed using a two-step ‘primer extension’ procedure, based on microsatellite-amplified fragment length poly- morphism (M-AFLP) technique. In the first step, highly enriched SSR gel profiles were pro- duced and, from the derived sequences of selected bands, forward primers directed towards the microsatellite motif were designed. In the second step, the opposite microsatellite flanking sequence was isolated using a nested approach on a restricted-ligated genomic fraction. Poly- morphism was explored in 24 plants of wild cardoon (Cynara cardunculus L. var. sylvestris) as well as two accessions of both globe artichoke (Cynara cardunculus L. var. scolymus), and cultivated cardoon (Cynara cardunculus L. var. altilis).
M-AFLP-based protocol for microsatellite loci isolation in Cynara cardunculus L. (Asteraceae)
ALBERTINI, Emidio;
2005
Abstract
Nine microsatellite markers for Cynara cardunculus L. were developed using a two-step ‘primer extension’ procedure, based on microsatellite-amplified fragment length poly- morphism (M-AFLP) technique. In the first step, highly enriched SSR gel profiles were pro- duced and, from the derived sequences of selected bands, forward primers directed towards the microsatellite motif were designed. In the second step, the opposite microsatellite flanking sequence was isolated using a nested approach on a restricted-ligated genomic fraction. Poly- morphism was explored in 24 plants of wild cardoon (Cynara cardunculus L. var. sylvestris) as well as two accessions of both globe artichoke (Cynara cardunculus L. var. scolymus), and cultivated cardoon (Cynara cardunculus L. var. altilis).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
