BACKGROUND: Mobilizing agents (MAs) have been suggested to improve the fungal degradation of polycyclic aromatichydrocarbons (PAHs) in soil. Three different MAs (Tween 20, Tween 80 and soybean oil) were investigated for their ability tostimulate contaminant degradation by either Phlebia sp. DABAC 9 or Allescheriella sp. DABAC1 in a soil spiked with a mixture ofPAHs.RESULTS: Phlebia sp. and Allescheriella sp. markedly differed in their growth capabilities under non-sterile conditions andwithout MAs (3.0 versus 0.1 µg ergosterol g−1soil, respectively). However, soybean oil led to a 35-fold increase of Allescheriellasp. growth. Contaminant degradations by Phlebia sp. DABAC 9 and Allescheriella sp. DABAC 1 were best supported by soybeanoil and Tween 20, respectively. Enumeration of cultivable bacteria and denaturing gradient gel electrophoresis (DGGE) analysisof PCR-amplified 16S rRNA showed that microbial density and biodiversity were positively affected by the mycoremediationespecially with Allescheriella sp., the use of which led to an evident detoxification.CONCLUSIONS: Allescheriella sp. DABAC 1 appears to be a promising strain in the remediation of PAH-contaminated soils. Thedifferent response of the two fungi to MAs addition confirms the stringent need for a preliminary lab-scale assessment offungus/MA combinations prior to application.
Effect of mobilizing agents on mycoremediation and impact on the indigenous microbiota
GIUBILEI, MARIA ANGELA;FEDERICI, Ermanno;Covino, Stefano;
2009
Abstract
BACKGROUND: Mobilizing agents (MAs) have been suggested to improve the fungal degradation of polycyclic aromatichydrocarbons (PAHs) in soil. Three different MAs (Tween 20, Tween 80 and soybean oil) were investigated for their ability tostimulate contaminant degradation by either Phlebia sp. DABAC 9 or Allescheriella sp. DABAC1 in a soil spiked with a mixture ofPAHs.RESULTS: Phlebia sp. and Allescheriella sp. markedly differed in their growth capabilities under non-sterile conditions andwithout MAs (3.0 versus 0.1 µg ergosterol g−1soil, respectively). However, soybean oil led to a 35-fold increase of Allescheriellasp. growth. Contaminant degradations by Phlebia sp. DABAC 9 and Allescheriella sp. DABAC 1 were best supported by soybeanoil and Tween 20, respectively. Enumeration of cultivable bacteria and denaturing gradient gel electrophoresis (DGGE) analysisof PCR-amplified 16S rRNA showed that microbial density and biodiversity were positively affected by the mycoremediationespecially with Allescheriella sp., the use of which led to an evident detoxification.CONCLUSIONS: Allescheriella sp. DABAC 1 appears to be a promising strain in the remediation of PAH-contaminated soils. Thedifferent response of the two fungi to MAs addition confirms the stringent need for a preliminary lab-scale assessment offungus/MA combinations prior to application.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
