Long-term somatic embryogenic callus has been obtained from in vitro anther culture of the cultivar Mandarino Tardivo di Ciaculli (MTC) N.L. 19 of Citrus reticulata Blanco. Somatic embryos obtained from these calli were employed for encapsulation in sodium alginate matrix, enriched of artificial endosperm. In order to evaluate the conversion ability in ex vitro conditions of the synthetic seeds, Plant Preservative Mixture (PPM™) and Thiophanate-methyl (TM), alone or in combination, were added to alginate matrix and to sowing substrate during conversion. The preliminary results showed that the fungicide TM and the germicide PPM allowed the conversion in ex vitro conditions at level ranging from 20.0 to 37.5% (respectively for TM and PPM in combination and TM alone), while synthetic seeds sown in sterile conditions and no submitted to treatment with TM and PPM (control) showed 35.0% of conversion. Although preliminary, the results reported conversion of MTC synthetic seeds in non-sterile conditions, showing interesting applications of the encapsulation technology in the nursery activity.
Preliminary results on ex-vitro conversion of encapsulated somatic embryos of citrus reticulata blanco (cv. Mandarino di Ciaculli).
MICHELI, Maurizio;STANDARDI, Alvaro
2005
Abstract
Long-term somatic embryogenic callus has been obtained from in vitro anther culture of the cultivar Mandarino Tardivo di Ciaculli (MTC) N.L. 19 of Citrus reticulata Blanco. Somatic embryos obtained from these calli were employed for encapsulation in sodium alginate matrix, enriched of artificial endosperm. In order to evaluate the conversion ability in ex vitro conditions of the synthetic seeds, Plant Preservative Mixture (PPM™) and Thiophanate-methyl (TM), alone or in combination, were added to alginate matrix and to sowing substrate during conversion. The preliminary results showed that the fungicide TM and the germicide PPM allowed the conversion in ex vitro conditions at level ranging from 20.0 to 37.5% (respectively for TM and PPM in combination and TM alone), while synthetic seeds sown in sterile conditions and no submitted to treatment with TM and PPM (control) showed 35.0% of conversion. Although preliminary, the results reported conversion of MTC synthetic seeds in non-sterile conditions, showing interesting applications of the encapsulation technology in the nursery activity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
